Sequences from confirmation amplicon


Confirmation sequences for line 057H09 and hit At2g38880 are shown.
Line ID 057H09
Locus At2g38880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16239916
Primers used for PCR BG03 - 8474, show primer details
   
Sequence Name 15-K021851-022-057-H09C-8409
GenBank Accession LN492775 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K021851-022-057-H09C-8409
AGCCATTTACATTGAATATATCCTGACTGTTGGTCTAGATTTATCTTTCTTGCTTCAAAA
ATTCTACACAAGACGTATTGAAGGGATTGTAAAACTGCTATCATGGCAAGCTTCGATGTT
ACTCACAGCTACTAATCTCTTGACTAGGATATTAAGGATTTCAGAACACATGATTAGGGA
GTCAAAGCAACATACCTCCCTGTACCTCGCTAGGTATATCTTTAGAGGTTCCAGGTAATC
CTCAAATCCTAATGTTGCCATTGCCCACAACAAATCATCACCATTCACAGTTTTCCTTTT
CTCTTTTTGACACTTATCACTGGCCCTGATTAGCAGAAACAGATACCAGTCAATTGGCCA
TTACAGTCAACGAATCAACATAAGACCAGCAAAAGAGACTTAAGCGCAACATCAGACAAG
CTTATCTTGAACCAATAGATAAATCATAGTTTCATACATGATAACTTATTTCTTCCTAAC
AAAGAACGGGGGCATCTGATGTTATAAGAAACATAAAACTCATCAATCAGAGACCTAAAA
CGCATTCAACACAAGCAATTCCTTCATGATGAAAAACATATCTTTTGCCTTAAATGAACA
AAAAGGAACAAAAACAAACAGATCCTAAAGATAAAAGAATCTTTGAAACATGATCAAGAT
ACGGCTTTTA
   
Sequence Name 16-K021851-022-057-H09C-BG03
GenBank Accession LN493245 [GenBank]
Sequencing primer gene specific primer, BG03: 5'-ACCATCTTAGGGTATAAAAGCCGT-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K021851-022-057-H09C-BG03
TCTTTTATCTTTAGGATCTGTTTGTTTTTGTTCCTTTTTGTTCATTTAAGGCAAAAGATA
TGTTTTTCATCATGAAGGAATTGCTTGTGTTGAATGCGTTTTAGGTCTCTGATTGATGAG
TTTTATGTTTCTTATAACATCAGATGCCCCCGTTCTTTGTTAGGAAGAAATAAGTTATCA
TGTATGAAACTATGATTTATCTATTGGTTCAAGATAAGCTTGTCTGATGTTGCGCTTAAG
TCTCTTTTGCTGGTCTTATGTTGATTCGTTGACTGTAATGGCCAATTGACTGGTATCTGT
TTCTGCTAATCAGGGCCAGTGATAAGTGTCAAAAAGAGAAAAGGAAAACTGTGAATGGTG
ATGATTTGTTGTGGGCAATGGCAACATTAGGATTTGAGGATTACCTGGAACCTCTAAAGA
TATACCTAGCGAGGTACAGGGAGGTATGTTGCTTTGACTCCCTAATCATGTGTTCTGAAA
TCCTTAATATCCTAGTCAAGAGATTAGTAGCTGTGAGTAACATCGAAGCTTGCCATGATA
GCAGTTTTACAATCCCTTCAATACGTCTTGTGTAGAATTTTTGAAGCAAGAAAGATAAAT
CTAGACCAACAGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATA
AAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAAT
TCGGAAATCTTTATNTCGACGTGTCTAC
Primers used for PCR BG04 - 8474, show primer details
   
Sequence Name 01-K022051-022-057-H09C-8409
GenBank Accession LN486397 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K022051-022-057-H07C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGACTAATATGTTTCAT
GTCCATGGCATTTACTCTCTCTTTTGTGCTTTTGTTGTTAACATACATAATAAAATCAAA
ATGTTTCTTATTAAGTTGAGACTAATTCGGTTCTTTTCATTCCCTGGGATTGGTTGCTGA
CACACCACCTGCTGATGCAGTTGGAGGTAAATAAACCACATACTTATTTCTACTTTATAT
TCCATTCCAGTGTTGCCCATTGGCTTCTTACGTTTTGTTGTGAAATTATGATAGGGTGAT
AATAAGGGATCAGGAAAGAGTGGAGATGGATCAAATAGAGATGCTGGTGGCGGTGTTTCT
GGTGAAGAAATGCCGGTATGATTTTGACAAGTATAGATGATACCAATGCTATACAGCCTA
TATTGTACTTAGCTCTGACTTCATCATTTTGATTCAATCACAGAGCTGGTAAAAGAAGTT
GCAAGTAGTGATTAAGAACAATCGCCAAATG
   
Sequence Name 02-K022051-022-057-H09C-BG04
GenBank Accession LN486852 [GenBank]
Sequencing primer gene specific primer, BG04: 5'-GAATTACTTGATCATTTGGCGATT-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K022051-022-057-H07C-BG04
CTTAATCACTACTTGCAACTTCTTTTACCAGCTCTGTGATTGAATCAAAATGATGAAGTC
AGAGCTAAGTACAATATAGGCTGTATAGCATTGGTATCATCTATACTTGTCAAAATCATA
CCGGCATTTCTTCACCAGAAACACCGCCACCAGCATCTCTATTTGATCCATCTCCACTCT
TTCCTGATCCCTTATTATCACCCTATCATAATTTCACAACAAAACGTAAGAAGCCAATGG
GCAACACTGGAATGGAATATAAAGTAGAAATAAGTATGTGGTTTATTTACCTCCAACTGC
ATCAGCAGGTGGTGTGTCAGCAACCAATCCCAGGGAATGAAAAGAACCGAATTAGTCTCA
ACTTAATAAGAAACATTTTGATTTTATTATGTATGTTAACAACAAAAGCACAAAAGAGAG
AGTAAATGCCATGGACATGAAACATATTAGTCAGGATATATTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAA
TGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAA
TAAACAAATTATTCTAATTCGGAAATCTTTATNTCGACGTGTCTACA

Last Updated on 06.12.2018 16:11