Sequences from confirmation amplicon


Confirmation sequences for line 800C02 and hit At2g39000 are shown.
Line ID 800C02
Locus At2g39000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16286794
Primers used for PCR 11ot - 8474, show primer details
   
Sequence Name 10-K114823-0022-800-C02C-11ot
GenBank Accession LN490775 [GenBank]
Sequencing primer gene specific primer, 11ot: 5'-AGATAGGTTAATGGCAATGGTGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K114823-0022-800-C02C-11ot
ATTTTAAAATTGGAGCTTTTGATGCAAAGATCAGCTTAAACAAAGGTTATGTTGCTGGAA
TCTTGACTGTTGATACTGTGGCTGATTACCTTCCAAGAAAAGGACCTCTCCGCCAGAGAA
GGTTCGCTTTTCGCCTTAGCTTTTATTTCTGTCGTCAAGCACTGTGCCTCTGTTCTTAAG
ACTAAGGACCTAGTCTCAAAAATGTGCAACTAGGATAACATAAAGTTTCGCAATCTTTAG
CTAATCTTGGTGAAAGAAGGATCTTGTCATTTTTTGTTGGGAATGTGTTGTGCTCGGATC
TGGATTCGTTTATTGTCTTATAAATGTAAATGTTGTGTCATGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATT
   
Sequence Name 04-K114822-0022-800-C02C-8409
GenBank Accession LN488024 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K114822-0022-800-C02C-8409
ACACAACATTTACATTTATAAGACAATAAACGAATCCAGATCCGAGCACAACACATTCCC
AACAAAAAATGACAAGATCCTTCTTTCACCAAGATTAGCTAAAGATTGCGAAACTTTATG
TTATCCTAGTTGCACATTTTTGAGACTAGGTCCTTAGTCTTAAGAACAGAGGCACAGTGC
TTGACGACAGAAATAAAAGCTAAGGCGAAAAGCGAACCTTCTCTGGCGGAGAGGTCCTTT
TCTTGGAAGGTAATCAGCCACAGTATCAACAGTCAAGATTCCAGCAACATAACCTTTGTT
TAAGCTGATCTTTGCATCAAAAGCTCCAATTTTGAAATCATCACTTCCAAAGCAGATGGT
TTCATCTACTGAACTACCTATCACAGCCACTAAACAAGTCCTCTGGCACCCAGGTGGAAT
AGAAAATCCCATCACCATTGC

Last Updated on 06.12.2018 16:11