Sequences from confirmation amplicon


Confirmation sequences for line 521B11 and hit At2g41850 are shown.
Line ID 521B11
Locus At2g41850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17463117
Primers used for PCR KQ60 - 8474, show primer details
   
Sequence Name 42-K101564-0022-521-B11C-8409
GenBank Accession LN504409 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K101564-0022-521-B11C-8409
TTGGACAAGTGTTTTACGTATATATTTTTTTACTTCAGGCTCTTACTTTTTACAACTCGA
AAAGTCTGATAGTGAAGAATCTGAAGGTGAGAAATGCACAGCAGATCCAGATTTCGATTG
AAAAATGCTCCAACGTTCAGGTCTCTAATGTCGTGGTAACTGCGCCTGCGGATAGTCCTA
ACACCGATGGTATTCATATCACTAACACCCAAAACATTCGAGTCTCCGAATCCATCATTG
GAACAGGTTACTATTTTATACATTAACATTTGTTTTCTTTATTTTGTCCGTGACTTAAAA
AAGTTGCTTGCTTACCAAGAGAGATGGATTTTAAGGCTATATATAATAATTTACGGAGAT
TGATCTAAAGGGAGTTTTAATAGTTGTTTTTTCCTAAATATAGGCGATGATTGCATATCT
NTTGAAAGTGGATCACAAAATGTTCAAATCAATGATATAACTTGCGGTCCCGGTCACGGT
ATCAGGTATCAAATCATTGTTTTGAATAATATATCAAAAGGGTTCGATCATAATCTAATA
CTAAGAAAATTTGATATTTATTTTTGAAAACATGAGCAGTATTGGGAGCCTTGGAGATGA
CAATTCAAAGGCTTTTGTCTCAGGCGTGACTGTGGATGGTGCTAAGCTTTCCGGTACAGA
CNATGGAGTAAGAATCAAAACTTACCAGGTGAAACTTCACAAACTAGTCCTTCGATTTTA
ACTAAACTCTCANAAATTTCCCCATCTGTTAATTGTTATTCCATTTAATCCAACCCAATT
TATCAGGGAGGGTCAGGAACTGCTAGCAATATNATATTTCANAACATTCAGATGGATNAT
GTTAAGAATCCGATCATAATCGACCAAGACTACGGCGACNAGAGC
   
Sequence Name 42-K101577-0022-521-B11C-KQ60
GenBank Accession LN504410 [GenBank]
Sequencing primer gene specific primer, KQ60: 5'-CTCTGTAGTGCATTTGCTCTTGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K101577-0022-521-B11C-KQ60
GATCGGATCTTACATTATCCTCTGAATGTTTTGAAATATAATATTGCTAGCAGTTCCTGA
CCCTCCCTGATAAATTGGGTTGGATTAAATGGAATAACAATTAACAGATGGGGAAATTTT
TGAGAGTTTAGTTAAAATCGAAGGACTAGTTTGTGAAGTTTCACCTGGTAAGTTTTGATT
CTTACTCCATTGTCTGTACCGGAAAGCTTAGCACCATCCACAGTCACGCCTGAGACAAAA
GCCTTTGAATTGTCATCTCCAAGGCTCCCAATACTGCTCATGTTTTCAAAAATAAATATC
AAATTTTCTTAGTATTAGATTATGATCGAACCCTTTTGATATATTATTCAAAACAATGAT
TTGATACCTGATACCGTGACCGGGACCGCAAGTTATATCATTGATTTGAACATTTTGTGA
TCCACTTTCAATAGATATGCAATCATCGCCTATATTTAGGAAAAAACAACTATNAAAACT
CCCTTTAGATCAATCTCCGTAAATTATTATATATAGCCTTAAAATCCATCTCTCTGGGTA
AGCAAGCAACTTTTTTAAGTCACGGACAAAATAAAGAAAACAAATGTTAATGTATAAAAT
AGTAACCTGTTCCAATGATGGATTCGGAGACTCGAATGTTTTGGGTGTTAGTGATATGAA
TACCATCGGTGTTAGGACTATCCGCAGGCGCAGTTACCACGACNTTAGAGACCTGAACGT
TGGAGCATTTTTCAATCGAAATCTGGATCTGCTGTGCATTTCTCACCTTCAGATTCTTCA
CTATCAGACTTTTCGAGTTGTAAAAAGTAAGAGCCTGAATGTAANANAATATATACGTAA
AACACTTGTCCAACTATNGCTTCATGTCCNGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAGAGTAATTACCAATTTTTTTTCATT

Last Updated on 06.12.2018 16:11