Sequences from confirmation amplicon


Confirmation sequences for line 134C04 and hit At2g44490 are shown.
Line ID 134C04
Locus At2g44490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18365703
Primers used for PCR vl01 - 8474, show primer details
   
Sequence Name 17-K016204-022-134-C04C-8409
GenBank Accession LN493650 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K016204-022-134-C04C-8409
TGTAGATTTCCCGGACATGAAGCCATTTACATTGTATATTTGAAATTTGAACAAGTTGTG
GAGATATTTATGTTAGGTGATGTGTATATGCAGAGATGACTTCAGAGACTATGCGAGTCT
CTGCTTCGAGAGATTTGGGGACAGAGTGAGTCTGTGGTGCACAATGAATGAGCCGTGGGT
CTACAGTGTCGCGGGCTATGACACAGGAAGGAAAGCGCCAGGACGGTGCTCCAAGTATGT
TAATGGCGCTAGTGTTGCTGGAATGTCGGGATACGAGGCATATATTGTGAGCCATAACAT
GCTTCTAGCGCACGCAGAAGCAGTGGAAGTGTTTAGAAAATGTGACCATGTAAGTTAAAA
AATTTCCCTTAATAGATAATATTTGTCATTTTCCAATACTATGTTCTGTTGTATTGATCT
GATTTGAATACAATGCAGATTAAAAACGGACAAATTGGGATTGCGCATAATCCACTTTGG
TACGAGCCATATGATCCGAGTGATCCAGATGATGTCGAAGGATGTAATCGAGCTA
   
Sequence Name 18-K016204-022-134-C04C-vl01
GenBank Accession LN494091 [GenBank]
Sequencing primer gene specific primer, vl01: 5'-CATGAAGTCCATAGCTCGATTACA-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K016204-022-134-C04C-vl01
CGACATCATCTGGATCACTCGGATCATATGGCTCGTACCAAAGTGGATTATGCGCAATCC
CAATTTGTCCGTTTTTAATCTGCATTGTATTCAAATCAGATCAATACAACAGAACATAGT
ATTGGAAAATGACAAATATTATCTATTAAGGGAAATTTTTTAACTTACATGGTCACATTT
TCTAAACACTTCCACTGCTTCTGCGTGCGCTAGAAGCATGTTATGGCTCACAATATATGC
CTCGTATCCCGACATTCCAGCAACACTAGCGCCATTAACATACTTGGAGCACCGTCCTGG
CGCTTTCCTTCCTGTGTCATAGCCCGCGACACTGTAGACCCACGGCTCATTCATTGTGCA
CCACAGACTCACTCTGTCCCCAAATCTCTCGAAGCAGAGACTCGCATAGTCTCTGAAGTC
ATCTCTGCATATACACATCACCTAACATAAATATCTCCACAACTTGTTCAAATTTCAAAT
ATACAATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTC
GTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTT
Primers used for PCR VL01 - 8474, show primer details
   
Sequence Name 49-K118301-0022-134-C04C-VL01
GenBank Accession LN506981 [GenBank]
Sequencing primer gene specific primer, VL01: 5'-CTCTTTGGAACTGCTTCATCTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K118301-0022-134-C04C-VL01
GACTATAAAAACATTACAAGATCAAAATGATATTTACTTTTAAGTTTTGTTTTTTTCTTT
GTATTAGTACGAAGGAGCAGTGAATGAAGGTGCGAGAGGACAAAGCGTGTGGGATCATTT
CTCCAACAGGTTTCCTCACAGAATCAGTGATTCTAGCGACGGAAACGTTGCCGTTGATTT
CTACCATCGTTACAAGGTACTTTTCTTCTCTTCTTGTTCTGTTTTTTTTTTCGATTTCTT
ACTTGTCAAGGTAATTTAGTTACAAGACTTGAAGATCTGTGATTGATTTCTTGTACAGGA
AGATATTAAGAGAATGAAGGATATAAACATGGATTCGTTTCGGCTTTCCATTGCTTGGCC
ACGAGTTCTACCTTGTAAGTATATCATCAGTTTCGAAACCTTATTTTAACGACAATCGAT
CGCCGTTTCAAAATCGATACTAATCTTTGAAAACAAAAACAGATGGCAAAAGGGATAGAG
GAGTTAGTGAAGAAGGAATTAAGTTTTACAATGATGTTATTGATGAACTCTTAGCCAATG
AAATCACTCCTCTGGTTACTATCTTTCATTGGGACATACCACAGGATCTTGAAGATGAAT
ACGGCGGTTTTCTAAGCGATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAAATGT
CCGC
   
Sequence Name 17-K118300-0022-134-C04C-8409
GenBank Accession LN494056 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K118300-0022-134-C04C-8409
AACCGCCNTATTCATCTTCAGATCCTGTGGTATGTCCCAATGAAAGATAGTAACAAGAGG
AGTGATTTCATTGGCTAAGAGTTCATCAATAACATCATTGTAAAACTTAATTCCTTCTTC
ACTAACTCCTCTATCCCTTTTGCCATCTGTTTTTGTTTTCAAAGATTAGTATCGATTTTG
AAACGGCGATCGATTGTCGTTAAAATAAGGTTTCGAAACTGATGATATACTTACAAGGTA
GAACTCGTGGCCAAGCAATGGAAAGCCGAAACGAATCCATGTTTATATCCTTCATTCTCT
TAATATCTTCCTGTACAAGAAATCAATCACAGATCTTCAAGTCTTGTAACTAAATTACCT
TGACAAGTAAGAAATCGAAAAAAAAAACAGAACAAGAAGAGAAGAAAAGTACCTTGTAAC
GATGGTAGAAATCAACGGCAACGTTTCCGTCGCTAGAATCACTGATTCTGTGAGGAAACC
TGTTGGAGAAATGATCCCACACGCTTTGTCCTCTCGCACCTTCATTCACTGCTCCTTCGT
ACTAATACAAAGAAAAAAACAAAACTTAAAAGTAAATATCATTTTGATCTTGTAATGTTT
TTTTATAGTTCTTGATTCGAACTGATTTACCTGATAAGAAGATGAAGCATTCCCC

Last Updated on 06.12.2018 16:11