Sequences from confirmation amplicon


Confirmation sequences for line 366H12 and hit At2g46410 are shown.
Line ID 366H12
Locus At2g46410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19049462
Primers used for PCR 25gp - 8474, show primer details
   
Sequence Name 29-K112477-0022-366-H12C-25gp
GenBank Accession LN499356 [GenBank]
Sequencing primer gene specific primer, 25gp: 5'-GTCGGTCGTTTATTTATGCATTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K112477-0022-366-H12C-25gp
TCTACTTCCGAGCTGTCATGACTGTGTTCAGCCTGATTTTTGATCTTGTTATTATTCTCT
GTTTTTTGTTTTAGTTGTTCAAATAGCAAAACCTAATCAAGATTTCGTTTTCAGTTTCTT
TTTTTATATATGATTCTTTAGCAAAACATATTCTTAATTTATGTCAGAACTCACTTTGGC
TAGTTTGGTTCAATTTTGATTACAGCATGTTTGTATGAAGTCAAAGTGTAAATTACGATT
TTGGTTCGGTTCCATAGAATTTTAACCGAATTACAAACTTTATGCGGTTTTTATCGGAAT
AAAAGGTATTTGGTTAAGTGTAAGTTCCTCAACACTGGAGAATCCCGGAAATATTCAATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCACAGTTATTA
   
Sequence Name 06-K112476-0022-366-H12C-8409
GenBank Accession LN488880 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K112476-0022-366-H12C-8409
ATTTACATTGAATATTGAATATTTCCGGGATTCTCCAGTGTTGAGGAACTTACACTTAAC
CAAATACCTTTTATTCCGATAAAAACCGCATAAAGTTTGTAATTCGGTTAAAATTCTATG
GAACCGAACCAAAATCGTAATTTACACTTTGACTTCATACAAACATGCTGTAATCAAAAT
TGAACCAAACTAGCCAAAGTGAGTTCTGACATAAATTAAGAATATGTTTTGCTAAAGAAT
CATATATAAAAAAAGAAACTGAAAACGAAATCTTGATTAGGTTTTGCTATTTGAACAACT
AAAACAAAAAACAGAGAATAATAACAAGATCAAAAATCAGGCTGAACACAGTCATTGACA
GCTCGGTAAGTTAGACCCAATAATATAATAGAAAAACCAAGAAAATGCATAAATAAACGA
CCGA

Last Updated on 06.12.2018 16:11