Sequences from confirmation amplicon


Confirmation sequences for line 406G09 and hit At2g46680 are shown.
Line ID 406G09
Locus At2g46680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19166870
Primers used for PCR 30xk - 8474, show primer details
   
Sequence Name 34-K119444-0022-406-G09C-30xk
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 30xk: 5'-GTTTTCTGATTCATGATGTGTGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K119444-0022-406-G09C-30xk
CTTTCTCCTCCTGTGTCTTCTTTTGCGTCGCCTCTTTTAGCCTCTGCAACTACATAAAGG
TATAAAACAACTCATCAACAATCTATTCTCTCCTTCAATAAACCATCAAAACAGAGTTTT
TCTTGATCACCTAATCAGTCCTTAATTGCAATATAACCACTAAAGCACATATTTTATCAC
ATATTTAAAACCACAGTTTTTCAGTATACAACCACAAAATACACAGATTTTAACTAACCA
TGTAAGAATAAACAGAGTTTAGAACATACCTCAGAGACTAAAGCTTGTTTTTCTTTCTTT
AAGGACTCGAACTGAGAAGCCAAGTTGTCGTAGTTTTGTCTGAGAATGTTGTACTCAGTC
TCGAGCTGCTTGGATTTCCAACGAGCCCTCTTGTTCTGAAACCATATAGCCACTTGCCTC
GGCTGCAACCCTAGCTCTCTAGCTAATTGAACCTTCTTCCTTGGCTCAAGCCTTGTCTCA
GACTCAAACATCATCTCCAGTGACTTGATCTGCTCGTCGCTAAACCTTCTCTGATTGTTC
TTGTTGTGGTTGCTCTTCTTCATCTTCTTCATGGTCAAGAATGGCTCTGCTGACATCATC
GCCGGAGAATATTCTCCACCTTCTGTCATCTCATCGGAATTTTTCCTCAGAGGATTTATG
TTTTCTTTATTTTTATTTTTTGGAATCTCTATGTTTGTGTGATGTTGGCTATCTATATAT
GTGTGTTGATGAGGCTAAGGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
   
Sequence Name 02-K119443-0022-406-G09C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K119443-0022-406-G09C-8409
CGGACTTGCCTCTCACACCATATATAGATAGCCAACATCACACAAACATAGAGATTCCAA
AAAATAAAAATAAAGAAAACATAAATCCTCTGAGGAAAAATTCCGATGAGATGACAGAAG
GTGGAGAATATTCTCCGGCGATGATGTCAGCAGAGCCATTCTTGACCATGAAGAAGATGA
AGAAGAGCAACCACAACAAGAACAATCAGAGAAGGTTTAGCGACGAGCAGATCAAGTCAC
TGGAGATGATGTTTGAGTCTGAGACAAGGCTTGAGCCAAGGAAGAAGGTTCAATTAGCTA
GAGAGCTAGGGTTGCAGCCGAGGCAAGTGGCTATATGGTTTCAGAACAAGAGGGCTCGTT
GGAAATCCAAGCAGCTCGAGACTGAGTACAACATTCTCAGACAAAACTACGACAACTTGG
CTTCTCAGTTCGAGTCCTTAAAGAAAGAAAAACAAGCTTTAGTCTCTGAGGTATGTTCTA
AACTCTGTTTATTCTTACATGGTTAGTTAAAATCTGTGTATTTTGTGGTTGTATACTGAA
AAACTGTGGTTTTAAATATGTGATAAAATATGTGCTTTAGTGGTTATATTGCAATTAAGG
ACTGATTAGGTGATCAAGAAAAACTCTGTTTTGATGGTTTATTGAAGGAGAGAATAGATT
GTTGATGAGTTGTTTTATACCTTTATGTAGTTGCAGAGGCTAAAAGAGGCGACGCAAAAG
AAGACACAGGAGGAGGAAAGGCAGTGTAGTGGAGATCAAGCGGTGGTTGCTCTAAGCAGC
ACACATCAT

Last Updated on 06.12.2018 16:11