Sequences from confirmation amplicon


Confirmation sequences for line 031A11 and hit At2g46790 are shown.
Line ID 031A11
Locus At2g46790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19234490
Primers used for PCR 51mm - 8474, show primer details
   
Sequence Name 62-K114191-0022-031-A11C-51mm
GenBank Accession LN510813 [GenBank]
Sequencing primer gene specific primer, 51mm: 5'-AATAATTTTGAAGGTAAATAAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K114191-0022-031-A11C-51mm
CCCGTCGGTCTTTGGGAGCCCTCTCGGGACTTCTTCCACCGCCTTAAAGCTCTCCCCTCC
CTGGGCTATATCCTCCTCTTGGAAGTCCCCTCTTTGAAAACAAAATTGTCTTGAAAAAAA
AACTAGGGTTGAAAAATAAAAAAAAGCGAGAGATAGAGAGTTTTGATTTGTCAAAGGTTT
TAAAAAAAGCGTTGAAACTCCCACTAATTATTTTTTTATAACCAAAGAAAAGGAAACTGA
TAATCCCAATTAACGGTCACAAAATCTTATTTTAACGGTCACAAAAAAAAATTTCTTTGT
GGATTTTTCTTTCAATAGCTTTGGTTCCCTTTTTTGGTTCCCCGGACTGTGAAAAAATTA
AGGGGATGATTTTAAATACATAGACTATAGCAAGGGTATATAAATCAAGTTTTATAAAAT
CTGTTTCTTGTGGGTATGTATCGAAAAAAAAGCGGCCTGGATATGATCCCAACAAAAAAT
ATTTCTCAACTTAACAAATTTGAATTTACGGTTTAAGCTTACCCGGATCATTTGCAAACA
TATATACAAAAGTTGCCCCAGTATCTCATATTACCTGAAAAAAAAAACCCATTCAAATAC
TTAAAAAAAAAAGCCCCCTTAAAATTGGCAAAGCCAAAAAAATTTTTGTTTTTAAATCCA
CCAAAAAAATTCAAAGAAAAACTGGAAAAACCTCTTCATATTTTTGAAAACCCTTCAAAT
TTCAGGTTTCCCAAAAA
   
Sequence Name 27-K114190-0022-031-A11C-oriL
GenBank Accession LN498488 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K114190-0022-031-A11C-oriL
GCAGATTCCCCATACCGGTAGAATAAATCGCGAGAAACCATTTAGCAAGGAAGTAGAAGC
TGGTTCTCAAAGCACCAATGAGGGGATTGCTGGACAAAGCAGTAGCACAGAGAAACCAAA
GGAAGAAGAAAGTGCGAAACAACGTTGGAGTAGAAGCCAGAGAGAAGCTGCATTGATGAA
GTTCCGGTTGAAGAGGAAAGATCGATGCTTTGACAAAAAGGTAAGAGACACACAAGCTTC
ATCATAAAAACAGCATCTGAAACAAATACAAGATCAAACTGATTCAATGGAGATGTATAA
AAATGGATTACAGGTTCGGTACCAGAGCAGGAAGAAGCTAGCAGAACAACGTCCTCGAGT
GAAAGGCCAGTTCGTGCGAACCGTGAATTCAGACGCGTCTACAAAATCATGAAGAGCTCT
TTCAGTTTTTCCTCTGATTTCTTTTGTTGATTTTATAACAGAAACTTATTTAGCTTTTGC
AATCTTTAGTGTGCTTTTTTTTTTTATGTATTTGATGGGCTTCTTCTTCTCAGCTAATAT
GAAATACTGGGGCAACTTTTGTATATATGTCTGCAAATGATCAGGCTAAGCTAAAACAGT
AATTCCAATTTCGTTAAGTTGAGAAATATTTCTTGTTGTGATCATATCCGGGCCGCTTCT
CTCTCGATACATACCAACAAGAAACAGATTCTATAAAACTTGATTTATATACCATTGCTA
TAGTCTATGTATTAAAACTCATCACATTAATTCTCTCCCGGTCAGAGAAACAAAAAAGGG
AAACAAAAGCTATCGAAAGAAAAATCAACAAAGAAATCTTTTTTTGTGACCGTTAAACTA
AAATT

Last Updated on 06.12.2018 16:11