Sequences from confirmation amplicon


Confirmation sequences for line 403C08 and hit At2g47750 are shown.
Line ID 403C08
Locus At2g47750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19562015
Primers used for PCR CB59 - 8474, show primer details
   
Sequence Name 17-K106138-0022-403-C08C-8409
GenBank Accession LN493860 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K106138-0022-403-C08C-8409
GAGCCATTTACATTGAATATATCCACGGACAGTACCTCTTATGAGTGTTAGGTACTGTAT
GTACTAATTTCCATATGTTAGTGGTACAATGATTATGAAACCATAGTCACACTGAGGTAA
GAAAATTGATAATTCTTTCAAGATTTTCAATAGTAATGGATAACATTGCAGAATTTGATG
TGATGTCCTTCCATTTCCATAGATTGGCTTTGGAGACATATCCATTATCCTACAACTAGA
GGAACAGTCCTAGTGGTTCTTTTATGGAATATGAGATCACCCATTTTTCTTTGTTTTGAG
CATGATGGTCACTTATTAAGTAAATACTTATGTGCACACAAGACGTATATCTCTTTTAAC
TCTAAAGATTTCAGAAAGTAGAAAAAGAGCCTGACTTGCCCTTAGGATTTTAAACAGCAT
CTAGGAGAACTAATTATTAGAGGAATCAAAGGAGGAATTCACTGACTTGTTCACGATTGG
AATGATAAGATTGTAGAGAAAAGTGCGGCGATCAAGATCTTCAGGAATGGTTGGCATTAG
CTTTGGCTCTCCAGCAGAAGTCCCAGAGCTGCGAAATGCAAGAAAGCGATTACTT
   
Sequence Name 17-K106137-0022-403-C08C-CB59
GenBank Accession LN493859 [GenBank]
Sequencing primer gene specific primer, CB59: 5'-AGCTGATGAAAATGTAATCGCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K106137-0022-403-C08C-CB59
AGCTAATGCCACCATTCCTGAAGATCTTGATCGCCGCACTTTTCTCTACAATCTTATCAT
TCCAATCGTGAACAAGTCAGTGAATTCCTCCTTTGATTCCTCTAATAATTAGTTCTCCTA
GATGCTGTTTAAAATCCTAAGGGCAAGTCAGGCTCTTTTTCTACTTTCTGAAATCTTTAG
AGTTAAAAGAGATATACGTCTTGTGTGCACATAAGTATTTACTTAATAAGTGACCATCAT
GCTCAAAACAAAGAAAAATGGGTGATCTCATATTCCATAAAAGAACCACTAGGACTGTTC
CTCTAGTTGTAGGATAATGGATATGTCTCCAAAGCCAATCTATGGAAATGGAAGGACATC
ACATCAAATTCTGCAATGTTATCCATTACTATTGAAAATCTTGAAAGAATTATCAATTTT
CTTACCTCAGTGTGACTATGGTTTCATAATCATTGTACCACTAACATATGGAAATTAGTA
CATACAGTACCTAACACTCATAAGAGGTACTGTCCGTGGATATATTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATTC
Primers used for PCR 41wq - 8474, show primer details
   
Sequence Name 54-K118893-0022-403-C08C-41wq
GenBank Accession LN508640 [GenBank]
Sequencing primer gene specific primer, 41wq: 5'-TCCAAAGTAAGTCTCAGAGGAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K118893-0022-403-C08C-41wq
ATCTTCCCTGGCTAAAACTCAAGTGCAGGTATGTATTGAGCCATTGAGCCCGTCACTACT
GCTTCGATGAACTTTGCCTTAGGCCAGAGCTGACACAGAATCCCTTTCCACGACGACCGC
CCACAGATCTCTTCGATCTCGCTAGCTAACTCTGGATTCGGCGACATTAAAAGACAAGAC
ATTGCCATTTGGCAACCTGGATCGGTGATCATGGGGTTGAGACTACCGGTGCGGATGTCT
TCACAGAGCTGACTCCATTTCTTCTCGAGATAAGAGATTGCACGGAGAAACGCTGAAGCA
AAGACTGCTCCTAAACGCATCACCTTGTGACGGTGAATCAGACCGGCTAGTAACTGACAG
TACATGCTTTGGTTGCTGTCTTCACAAAGGATGGTTTGGATAGGACTGGTTAAGTCGTTG
AATGGATCATAAGGTCTGCACTGGAAATGCTTGCTTTTGTAGTAACTAGTGAGGACAGCT
CTGATTGGTAAACCACAAGGAGTTGATGTTTCAGCCTTTACGAAATTTAGGTACATTGCT
TTTCCTTTGTCAAGTCCTGTTATGTACCTGTTGTAAAAAGGTTAAAAACAGAGCAAGAAG
AAGATGGTAAAAGAGGTAACCTCAACGGATATGAAAGATTCTTTAGGTTTAGTGAGTGCA
TGTTCAAAATTCTTAGAGAATCTTTAGTGGGGACATCAAAATTGGCGTGTGGATAAAAGA
TGATGGAAGCAAACTTTATAGTACATGACATGATACCACTAATTGTCCCCAAAAAAAACA
AAAACTTTAAGAAGAAAGCAAACTTCATTTTCTTCCTAAGTGCGAAACCCATTGTGATGA
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGTTCAATATGG
AGAAAAAGAAG
   
Sequence Name 14-K118892-0022-403-C08C-8409
GenBank Accession LN492737 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K118892-0022-403-C08C-8409
ATTTTCTCACAATGGGTTTCGCACTTAGGAAGAAAATGAAGTTTGCTTTCTTCTTAAAGT
TTTTGTTTTTTTTGGGGACAATTAGTGGTATCATGTCATGTACTATAAAGTTTGCTTCCA
TCATCTTTTATCCACACGCCAATTTTGATGTCCCCACTAAAGATTCTCTAAGAATTTTGA
ACATGCACTCACTAAACCTAAAGAATCTTTCATATCCGTTGAGGTTACCTCTTTTACCAT
CTTCTTCTTGCTCTGTTTTTAACCTTTTTACAACAGGTACATAACAGGACTTGACAAAGG
AAAAGCAATGTACCTAAATTTCGTAAAGGCTGAAACATCAACTCCTTGTGGTTTACCAAT
CAGAGCTGTCCTCACTAGTTACTACAAAAGCAAGCATTTCCAGTGCAGACCTTATGATCC
ATTCAACGACTTAACCAGTCCTATCCAAACCATCCTTTGTGAAGACAGCAACCAAAGCAT
GTACTGTCAGTTACTAGCCGGTCTGATTCACCGTCACAAGGTGATGCGTTTAGGAGCAGT
CTTTGCTTCAGCGTTTCTCCGTGCAATCTCTTATCTCGAGAAGAAATGGAGTCAGCTCTG
TGAAGACATCCGCACCGGTAGTCTCAACCCCATGATCACCGATCCAGGTTGCCAAATGGC
AATGTCTTGTCTTTTAATGTCGCCGAATCCAGAGTTAGCTAGCGAGATCGAAGAGATCTG
TGGGCGGTCGTCGTGGAAAGGGATTCTGTGTCAGCTCTGGCCTAAGGCAAAGTTCATCGA
AGCAGTAGTGACGGGCTCAATGGCTCAATACATACCTGCACTTGAGTTTTTTAG

Last Updated on 06.12.2018 16:11