Sequences from confirmation amplicon


Confirmation sequences for line 446H09 and hit At3g02580 are shown.
Line ID 446H09
Locus At3g02580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:547868
Primers used for PCR PG23 - 8474, show primer details
   
Sequence Name 48-K101745-0022-446-H09C-8409
GenBank Accession LN506465 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K101745-0022-446-H09C-8409
TTCGGAAGNAGCCATTTACATTGAATATATCCTGACATTAAGCCTCTCTATAAGTATCTC
CATGCCACCCATCATATCTACAACAAGCAGAATACACTCTCTCCATTTGCCGGTAAGTGT
TTTCAGTTTGTTCTTCTTTAGTTCTTGTAAAAGATTGGTAGCATTTAGTTTCTTACCAGA
AAAGACTTTGTCAGCAGCTGCTTGTACTCCAAATCACATTTTGCATTCCTTATCCATAAA
GTAACCAGAAAGGCTAGAATTATATAAATGTCAGCTGCATTACTTCACATATGTCAGAGA
GACTTCTGACTTAACCAGAGTTTAGATCTTTGTGTTTCTCTTCTGGTCTCGGACTGATTG
GAAATGACGAGAAGTTCTTTTATCTACTTCCCTGGAGTGTATCTTGGTTAATCCAAGGAT
GTGACATCTAATATTACTTGTAACTTCCTTACGTTTTTGTTTACAGGGCTTGCATTTCAC
CCAGTAGACGGGATACTTCAGGCTGTACCGCAGGTGATAGCGCTGTTTATAGTGCCAATT
CATTTCACAACTCANATAGGTCTTTTGTTCATGGAAGCGATATGGACGGCGAACATCCAT
GACTGCATCCATGGCAACATCTGGCCAGTAATGGGTGCAGGATACCATACGATACACCAC
ACG
   
Sequence Name 48-K101751-0022-446-H09C-PG23
GenBank Accession LN506466 [GenBank]
Sequencing primer gene specific primer, PG23: 5'-ATGCTTGTATGTCGTGTGGTGTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K101751-0022-446-H09C-PG23
CATGGATGCAGTCATGGATGTTCGCCGTCCATATCGCTTCCATGAACAAAAGACCTATAT
GAGTTGTGAAATGAATTGGCACTATAAACAGCGCTATCACATGCGGTACAGCCTGAAGTA
TCCCGTCTACTGGGTGAAATGCAAGCCCTGTAAACAAAAACGTAAGGAAGTTACAAGTAA
TATTAGATGTCACATCCTTGGATTAACCAAGATACACTCCAGGGAAGTAGATAAAAGAAC
TTCTCGTCATTTCCAATCAGTCCGAGACCAGAAGAGAAACACAAAGATCTAAACTCTGGT
TAAGTCAGAAGTCTCTCTGACATATGTGAAGTAATGCAGCTGACATTTATATAATTCTAG
CCTTTCTGGTTACTTTATGGATAAGGAATGCAAAATGTGATTTGGAGTACAAGCAGCTGC
TGACAAAGTCTTTTCTGGTAAGAAACTAAATGCTACCAATCTTTTACAAGAACTAAAGAA
GAACAAACTGAAAACACTTACCGGCAAATGGAGAGAGTGTATTCTGCTTGTTGTAGATAT
GATGGGTGGCATGGAGATACTTATAGAGAGGCTTAATGTCAGGATATATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
ANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTA

Last Updated on 06.12.2018 16:11