Sequences from confirmation amplicon


Confirmation sequences for line 595B10 and hit At3g02590 are shown.
Line ID 595B10
Locus At3g02590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:550542
Primers used for PCR 74qa - 8474, show primer details
   
Sequence Name 46-K115550-0022-595-B10C-74qa
GenBank Accession LN505959 [GenBank]
Sequencing primer gene specific primer, 74qa: 5'-TGTCATCATATCTTGAGCAACCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K115550-0022-595-B10C-74qa
ATTTACAGTTTGGAGATTCAAAGATTACTGTGGTCTGTGGTAAAACTGACACCGCAACTG
ACCTATTAAACCCTTCAGATTTAGTCCAAATTTGAAACTCTTCATCCAGCAATTATATAA
TTGAGGTTAATAATCATCAGAGATTTTTAGCAATAAAAGTCTGAAACTTTGCATCAAATT
ATGCAGTCATGTTTTTAATTCCAACAAAGTGATTAGAAAGGTTTTAATAAGAGTCACGAG
AAGAGCAACATATGAAGAATACATGTGAGCATTGAACATTCTCACTTTTCTTTATACATA
CATGTGGCTGGTATAATGCTTGTAAATGGGTGCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCG
   
Sequence Name 19-K115549-0022-595-B10C-8409
GenBank Accession LN494895 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K115549-0022-595-B10C-8409
CCATTTACAGCATTATACCAGCCACATGTATGTATAAAGAAAAGTGAGAATGTTCAATGC
TCACATGTATTCTTCATATGTTGCTCTTCTCGTGACTCTTATTAAAACCTTTCTAATCAC
TTTGTTGGAATTAAAAACATGACTGCATAATTTGATGCAAAGTTTCAGACTTTTATTGCT
AAAAATCTCTGATGATTATTAACCTCAATTATATAATTGCTGGATGAAGAGTTTCAAATT
TGGACTAAATCTGAAGGGTTTAATAGGTCAGTTGCGGTGTCAGTTTTACCACAGACCACA
GTAATCTTTGAATCTTCCAAACTTGTAAAATAAATAATTATACTCATATGGTAGGTGAGG
TTGCTCAAAAANTGATGAC

Last Updated on 06.12.2018 16:11