Sequences from confirmation amplicon


Confirmation sequences for line 052H12 and hit At3g02875 are shown.
Line ID 052H12
Locus At3g02875 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:632709
Primers used for PCR 03wc - 3144, show primer details
   
Sequence Name 95-K118393-0022-052-H12C-03wc
GenBank Accession LN515359 [GenBank]
Sequencing primer gene specific primer, 03wc: 5'-TGACTCGCTTGGAGTGAAGTATAA-3'
Sequence (A. th genome BLAST matches underlined) 
>95-K118393-0022-052-H12C-03wc
ATCGGATCCTGTTCGAACCTGTTTTCGGACTTAGAGCCGACATGGACGCACTTCCGTTAC
AGGTTTGAAATCTAATAATCAAAGTTCCGATTTTTACTCTGATTTTTAGATTGCTTTGTT
TGACTAATGAATTTGAAAAAATGGGGACAAAAAAAGGAATTAGTGGAATGGGAATCGAAA
AGTAAAGTAGATGGAAAGATGCATGCTTGTGGTCATGATACTCATGTTGCTATGCTTCTT
GGTGCTGCTAAGCTTCTTCAAACCACAAAACACCTCATCAAGGTCTCTCTTTTTTCTCTT
TCACTCAATTTATATGTATAATTTTGAATGATTCTGTCTGTGCCCTGCAATTAAAACTCA
TTTCGATGTGGGTTAGTGTTTTCTAAGATTCTTAGATATATGTGTGTGTATGTCTTTGAA
CTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTC
CAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGC
GTCATCCCTTACGTCAGTGGAGAT
   
Sequence Name 47-K118392-0022-052-H12C-3144
GenBank Accession LN506334 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K118392-0022-052-H12C-3144
CACTATCCTTCGCAGACCCTTCCTCATATAAGGAAGTTCATTTCATTTGGAGAGGACAGG
GTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTTCAAAGACAT
ACACACACATATATCTAAGAATCTTAGAAAACACTAACCCACATCGAAATGAGTTTTAAT
TGCAGGGCACAGACAGAATCATTCAAAATTATACATATAAATTGAGTGAAAGAGAAAAAA
GAGAGACCTTGATGAGGTGTTTTGTGGTTTGAAGAAGCTTAGCAGCACCAAGAAGCATAG
CAACATGAGTATCATGACCACAAGCATGCATCTTTCCATCTACTTTACTTTTCGATTCCC
ATTCCACTAATTCCTTTTTTTGTCCCCATTTTTTCAAATTCATTAGTCAAACAAAGCAAT
CTAAAAATCAGAGTAAAAATCGGAACTTTGATTATTAGATTTCAAACCTGTAACGGAAGT
GCGTCCATGTCGGCTCTAAGTCCGAAAACAGGTTTCGAACAGGATCCGATCCAAGCGACG
ACGCCNGTCTTCGCCACAGGATACTTATACTTC

Last Updated on 06.12.2018 16:11