Sequences from confirmation amplicon


Confirmation sequences for line 379C12 and hit At3g03530 are shown.
Line ID 379C12
Locus At3g03530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:844720
Primers used for PCR PD08 - 8474, show primer details
   
Sequence Name 39-K027488-022-379-C12C-8409
GenBank Accession LN503231 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K027488-022-379-C12C-8409
AGCCATTTACAATTGAATATATCCTGAATGGAGTGACCTGGATCCGGGTTAACGTACTGA
GACTGATCCCCGAAGACTACACGAAGAGAGTTTGTGTCGGATGACGAAACCGTGTTGGAT
TTGGGATCAGATTTCGTGACGCCGTCGATTTCCCGGTTTAGTTCTTTAAACCAGCCTAGG
GTGTGGTCAAAAGAACGATTTTCTTGAACCAAAACGACGATGGTTTTGATCGGATACGAT
CCTGAACCGCCTTTGGTCGTCTCGATCATTCCGTTTCTTTTCTTTTTCGAATGTTAAACG
TTTTGGGTAGAAACAATTCGTGGGGGTTATACTTATATAAATATATACGTAGTGATCATT
GGAGATTTGAAGAAGACGTAAACAGCAAA
   
Sequence Name 40-K027488-022-379-C12C-PD08
GenBank Accession LN503584 [GenBank]
Sequencing primer gene specific primer, PD08: 5'-ACGTGCATACTTATGATTTGCTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K027488-022-379-C12C-PD08
TATATATTTATATAAGTATAACCCCCACGAATTGTTTCTACCCTTAACGTTTAACATTCG
AAAAAGAAAAGAAACGGAATGATCGAGACGACCAAAGGCGGTTCAGGATCGTATCCGATC
AAAACCATCGTCGTTTTGGTTCAAGAAAATCGTTCTTTTGACCACACCCTAGGCTGGTTT
AAAGAACTAAACCGGGAAATCGACGGCGTCACGAAATCTGATCCCAAATCCAACACGGTT
TCGTCATCCGACACAAACTCTCTTCGTGTAGTCTTCGGGGATCAGTCTCAGTACGTTAAC
CCGGATCCAGGTCACTCCATTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACA
TTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATT
ATTCTAATTCGGAAATCTTTATTTCGACGTGTCTA

Last Updated on 06.12.2018 16:11