Sequences from confirmation amplicon


Confirmation sequences for line 343D11 and hit At3g04070 are shown.
Line ID 343D11
Locus At3g04070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1061951
Primers used for PCR BG12 - 8474, show primer details
   
Sequence Name 37-K101745-0022-343-D11C-8409
GenBank Accession LN502560 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K101745-0022-343-D11C-8409
CATTTACAATTGAATATATCCTGATATATAGACTTCTGGCGTTTACCGGGTTGATCATTT
TGCAAATTTGGCAAAAAGGTTCCGCGGTCTACGAATGGTTCGTTGTCATTTTCCTCATGC
TCTTGATTGCTTGTGACCAAAGCAACATCAGGTGATGACAATGAAGCGTGTGATTTCTTG
TAAATCCGGCAAAGAACCCAATCATCCAGCTGCATGAAATTAAGGAATATAAAAACCATA
AGTATTGGGGCAAAATAATAAAACAATCAAAGTAAGTGGCGATTGTGAAAACCAAAGAGA
TAAATAATGGTTTATTCCAGCCCTCTAATAACCGGTTTATGCCTTCCGGTTAAAATAGTT
GGCGATACCAAAAATACAAATTTTGAAACTGAAATCACCATAATAATAGTCTATATCTTT
AGTAGTAGCAAAAACTTACTCTCATCGAATATTCTTTAGAATTCCTATCTCCAAAATTAT
TATTAACTTCGCTACCACCGCTCCTAGAAGAGTTAATTCTTTTGGGAGATAATGAATCGG
CAAGACGATATTCATGCATGATCCAATTGGTTTTAACACCTTTTGGAGGCTTTCCTCTAT
AAAACACAAGAGCTTTTTTTATACCAATGTTTTCATGAAACCCTTCACCATTTGGTACCG
CAATCAATTTATCTGTTCCGGTTGCTTTCCAATATCCAGACGCAGCTGCTCTGTTTGGTC
TTGCTCCGTTTGGATATNTCCTATCCCTCGGACTGAAAAAATACCACTCTTCTTCCCCAA
   
Sequence Name 37-K101751-0022-343-D11C-BG12
GenBank Accession LN502561 [GenBank]
Sequencing primer gene specific primer, BG12: 5'-ATTTGGGGAGAAAGAGTGGTATTT-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K101751-0022-343-D11C-BG12
CAACGGAGCAGACCAAACAGAGCAGCTGCGTCTGGATATTGGAAAGCAACCGGAACAGAT
AAATTGATTGCGGTACCAAATGGTGAAGGGTTTCATGAAAACATTGGTATAAAAAAAGCT
CTTGTGTTTTATAGAGGAAAGCCTCCAAAAGGTGTTAAAACCAATTGGATCATGCATGAA
TATCGTCTTGCCGATTCATTATCTCCCAAAAGAATTAACTCTTCTAGGAGCGGTGGTAGC
GAAGTTAATAATAATTTTGGAGATAGGAATTCTAAAGAATATTCGATGAGAGTAAGTTTT
TGCTACTACTAAAGATATAGACTATTATTATGGTGATTTCAGTTTCAAAATTTGTATTTT
TGGTATCGCCAACTATTTTAACCGGAAGGCATAAACCGGTTATTAGAGGGCTGGAATAAA
CCATTATTTATCTCTTTGGTTTTCACAATCGCCACTTACTTTGATTGTTTTATTATTTTG
CCCCAATACTTATGGTTTTTATATTCCTTAATTTCATGCAGCTGGATGATTGGGTTCTTT
GCCGGATTTACAAGAAATCACACGCTTCATTGTCATCACCTGATGTTGCTTTGGTCACAA
GCAATCAAGAGCATGAGGAAAATGACAACGAACCATTCGTAGACCGCGGAACCTTTTTGC
CAAATTTGCAAAATGATCAACCCCTTANACGCCAGAAGTCTATATATCAGGATATATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGA

Last Updated on 06.12.2018 16:11