Sequences from confirmation amplicon


Confirmation sequences for line 280B04 and hit At3g04660 are shown.
Line ID 280B04
Locus At3g04660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1264774
Primers used for PCR 25xm - 8474, show primer details
   
Sequence Name 68-K119407-0022-280-B04C-25xm
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 25xm: 5'-CAGAGTGTAACTGACGCTACGATG-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K119407-0022-280-B04C-25xm
TTGGGACGGTACACTGAGAAAAGAAGTCGTGGATGAGCCAAAGATCGTCTCATGTACAAG
TCGGTGAAAACTTGGCCGCGGATTATCGATGACCAATGATTTGAAACGAAACCTAGCTTG
GCTATGGATTTCGGCGGTGACTTCATGAGAATCTCTATTTTCAGTTCCAGAGGAAGAATC
GAACTCGGGTCGTATTCAGCTCTTTTTTCTTCTGGTTTCTTAGACTTCCTTCCCCTTTTC
TTCATCGCGATTTCTCTTCTTCTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 28-K119406-0022-280-B04C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K119406-0022-280-B04C-8409
ATTACAATTGAATATATCCTGAGAAGAAGAGAAATCGCGATGAAGAAAAGGGGAAGGAAG
TCTAAGAAACCAGAAGAAAAAAGAGCTGAATACGACCCGAGTTCGATTCTTCCTCTGGAA
CTGAAAATAGAGATTCTCATGAAGTCACCGCCGAAATCCATAGCCAAGCTAGGTTTCGTT
TCAAATCATTGGTCATCGATAATCCGCGGCCAAGTTTTCACCGACTTGTACATGAGACGA
TCTTTGGCTCATCCACGACTTCTTTTCTCAGTGTACCGTCCCAACATGCAGATGCAGTTC
TTTCACTCCTGCTCTCAGGAAGATCCGTCTTCTGATCATCGTAGCGTCGTTAACACTCTG

Last Updated on 06.12.2018 16:11