Sequences from confirmation amplicon


Confirmation sequences for line 790G01 and hit At3g04770 are shown.
Line ID 790G01
Locus At3g04770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1310843
Primers used for PCR 11hd - 8474, show primer details
   
Sequence Name 42-K112477-0022-790-G01C-11hd
GenBank Accession LN504513 [GenBank]
Sequencing primer gene specific primer, 11hd: 5'-GATTGCTACAATAACCCTAGCAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K112477-0022-790-G01C-11hd
TCAGATTGATTATGTAAATACCTGACCAAATAAAAAAAAATCCAAACTTCATAAACAAAA
AAAAGATCCAAAAACATCACAAAACTCTATGAAACCGAGAATTACAGATGAATACCCAAC
ACTATGGAAACTGGAGAAACTATCAATTCGTAAGCTACGTGTAGATGAATGTGGAAACGT
TGCTTCTATATAGAATTACATGTACAATGAAATTTGGATGAAGATGAGATAGTTTACCGT
CGTCGCGTCTTTTGAAGACATAACGCTCCATCTGATAGTTGCAGTTTTTGGTACCAAGGT
GAACATCGGCTGATAACATCATCTGGATATCAGCTTCTTTCTCTGATACCTGACGTCCCG
CCGTCGCTACTCCGTTCGCCGCAATGAGTATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGNTTATT
   
Sequence Name 19-K112476-0022-790-G01C-8409
GenBank Accession LN494824 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K112476-0022-790-G01C-8409
TCGGATGAGCATTTCATTGAATACTCATTGCGGCGAACGGAGTAGCGACGGCGGGACGTC
AGGTATCAGAGAAAGAAGCTGATATCCAGATGATGTTATCAGCCGATGTTCACCTTGGTA
CCAAAAACTGCAACTATCAGATGGAGCGTTATGTCTTCAAAAGACGCGACGACGGTAAAC
TATCTCATCTTCATCCAAATTTCATTGTACATGTAATTCTATATAGAAGCAACGTTTCCA
CATTCATCTACACGTAGCTTACGAATTGATAGTTTCTCCAGTTTCCATAGTGTTGGGTAT
TCATCTGTAATTCTCGGTTTCATAGAGTTTTGTGATGTTTTTGGATCTTTTTTTTGTTTA
TGAAGTTTGGATTTTTTTTTATTTGGTCAGGTATTTACATAATCAATCTTGGAAAGACAT
GGGACAAGCTTCAGATGGCTGCTAGGGTTATTGTAGCAAT

Last Updated on 06.12.2018 16:11