Sequences from confirmation amplicon


Confirmation sequences for line 053A09 and hit At3g04940 are shown.
Line ID 053A09
Locus At3g04940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1367577
Primers used for PCR KS04 - 3144, show primer details
   
Sequence Name 55-K023371w-022-053-A09C-KS04
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, KS04: 5'-TCAAACTCAAAACTAAGTGTCCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K023371w-022-053-A09C-KS04
AGTTGTTGAGAAGCTTGGGTTTCAAAGAGAGGGTTTGCTCAGGAAGTAGACTTGTCTCAA
AGGTGAAACCTATGATGTGTTTGTTTATAGTCTCTTGTCCTCTGATCTTTTTCCTCTTCC
ACAATCCTCTAGCCAAATTGTCTACTACTAGTAACATGGGTTGAAATCCTACATGGCCAT
TATATAGTTCTAGATTCATTGTCAACAAAAGTTTTACAGACTTACACATATATTAGCTAG
AAAAAAACCACCAATAGAATGTCAAAGGACAGTCCACCTTAGAGGCTAGAGATTAGAGAT
CATACATCATCTGAAATCTTAAAGTTCATGAAACTATGACAGAATATAGATGTGCAATGA
TAGTTTAAGCCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAA
TGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCA
TCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACG
TCTTCTTTTTCCACGATGCTCCTCGTGGGTGG
   
Sequence Name 51-K023371w-022-053-A09C-3144
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K023371w-022-053-A09C-3144
GGGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCAT
TTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGCTTAA
ACTATCATTGCACATCTATATTCTGTCATAGTTTCATGAACTTTAAGATTTCAGATGATG
TATGATCTCTAATCTCTAGCCTCTAAGGTGGACTGTCCTTTGACATTCTATTGGTGGTTT
TTTTCTAGCTAATATATGTGTAAGTCTGTAAAACTTTTGTTGACAATGAATCTAGAACTA
TATAATGGCCATGTAGGATTTCAACCCATGTTACTAGTAGTAGACAATTTGGCTAGAGGA
TTGTGGAAGAGGAAAAAGATCAGAGGACAAGAGACTATAAACAAACACATCATAGGTTTC
ACCTTTGAGACAAGTCTACTTCCTGAGCAAACCCTCTCTTTGAAACCCAAGCTTCTCAAC
AACTCTTTGGGACACTT

Last Updated on 06.12.2018 16:11