Sequences from confirmation amplicon


Confirmation sequences for line 378C04 and hit At3g05850 are shown.
Line ID 378C04
Locus At3g05850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1745919
Primers used for PCR SR35 - 8474, show primer details
   
Sequence Name 44-K103544-0022-378-C04C-SR35
GenBank Accession LN505154 [GenBank]
Sequencing primer gene specific primer, SR35: 5'-TGCCTTTGTGAGAATATCTCCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K103544-0022-378-C04C-SR35
TCAATTCGGATGGATTAATAATAATCTGTGCATCTTCAGCTCGGTTCATGACTTCCTCCA
TAGTCACAGCGGGAGGAGCTGAGACTGAGATTGAGATGGGAAGCTCTTCTGGTCCCATTG
TGTCGTCAATCATGTCGTCCATAGGAACCGGGGGAATCGCTTCAGACAACGTTGTTCTGC
TTGATCTGGACGAAAAAAACTCTTAGAAACTTAAAAAAGATAAGGTGATATAATGATTTA
TGTAAAGGCTTTGCTAACCTGCTAGCTGGCATGTTTGAAATGTCGGGAGTAGCTTCTTCG
GGTAAGAGATAGACATCAGCAGTGTTGGAGTTCTCATGAAATTTGATCATCCGTTTCAAA
TCTTTATCGTTAGATATGGAGATGAGCGTTTTCTTGTTATCCGGGAGAAAGTATTTTAGA
GAAACTGTCCTGACATCGCAATTAAACATCTCACCAATTTCTGAGATAAAGTCTATGAAT
TTCATCTGCTCGTCCACATCTATCGCGTGAGCATCGCCGCCTTTGTAAGACAGCGACCCG
CCTTTCTCTGTCTGGAACTCCCCACCAGACATACATATTGCTATGACCCGCAGCGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 60-K103545-0022-378-C04C-8409
GenBank Accession LN510234 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K103545-0022-378-C04C-8409
CATATGTATGTCTGGTGGGGAGTTCCGACAGAGAAAGGCGGGTCGCTGTCTTACAAAGGC
GGCGATGCTCACGCGATAGATGTGGACGAGCAGATGAAATTCATAGACTTTATCTCAGAA
ATTGGTGAGATGTTTAATTGCGATGTCAGGACAGTTTCTCTAAAATACTTTCTCCCGGAT
AACAAGAAAACGCTCATCTCCATATCTAACGATAAAGATTTGAAACGGATGATCAAATTT
CATGAGAACTCCAACACTGCTGATGTCTATCTCTTACCCGAAGAAGCTACTCCCGACATT
TCAAACATGCCAGCTAGCAGGTTAGCAAAGCCTTTACATAAATCATTATATCACCTTATC
TTTTTTAAGTTTCTAAGAGTTTTTTTCGTCCAGATCAAGCAGAACAACGTTGTCTGAAGC
GATTCCCCCGGTTCCTATGGACGACATGATTGACGACACAATGGGACCAGAAGAGCTTCC
CATCTCAATCTCAGTCTCAGCTCCTCCCGCTGTGACTATGGAGGAAGTCATGAACCGAGC
TGAAGATGCACAGATTATTATTAATCCATCCGAATTGATGTCATCGATTGTTGAAGTCCC
GAATCCGAAAGGAGATATTCTCACAAAGGC

Last Updated on 06.12.2018 16:11