Sequences from confirmation amplicon


Confirmation sequences for line 072E02 and hit At3g06850 are shown.
Line ID 072E02
Locus At3g06850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2160617
Primers used for PCR DM14 - 3144, show primer details
   
Sequence Name 68-K101747-0022-072-E02C-3144
GenBank Accession LN511975 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K101747-0022-072-E02C-3144
TCCACTTCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACA
GGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCATCTGTTTGAT
ACTCGAACAGAAACAAACACACGGGTACGTAACATTTATCTTTTTAAGCTCTCTTCTTAG
AAACATCTTCCATCAACTCAATCTGTTTGTCTATTTCTTCTCCCTTGTACTGTTTTTTGT
TTTTTACCAAAGACCAACCGTTCATAACAATGATCGCGCGACGGATCTGGCGAAGCCACC
GGTTTCTCCGCCCATTCAGCTCGTCATCTGTTTGCTCTCCGCCGTTCCGGGTACCGGAGT
ATCTTTCTCAGTCGTCTTCCTCTCCGGCGTCGCGCCCATTCTTTGTTCACCCTCCCACTG
TATGTTTCTTCTCACCAAATTTCCTTCCTTTTTCAGTTTTTTTTTTCAAATTAATTTTGA
GGCATGACATTAGCTTCCGAGAAGCTCTTCTAGGCGGAAAAAGAAACATCGAAACTGTAT
AAATTTAGAAAGAAAATAAATTTATCAGAGAGACTTAAACTTGGAAAACTCTGTTTGCTT
AGAAGGTGTGGTTTTTCTGGTAATTTTTGGACAAAGGATCAAACAAGTTTGTCATATTTT
TCACAGTTGATGAAATGGGGTGGAGGAAGTAGAAGCTGGTTTTCGAACGAAGCCATGGCC
ACTGATTCAAATTCAGGGTTAATTGA
   
Sequence Name 68-K101753-0022-072-E02C-DM14
GenBank Accession LN511976 [GenBank]
Sequencing primer gene specific primer, DM14: 5'-TGAGCTAGTGGCACATCAATTAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K101753-0022-072-E02C-DM14
GAAACAGCTTCTACTTCCTCCCCCCATTTCATCAACTGTGAAAAATATGACAAACTTGTT
TGATCCTTTGTCCAAAAATTACCAGAAAAACCACACATTCTAAGCAAACAGAGTTTTCCA
AGTTTAAGTCTCTCTGATAAATTTATTTTCTTTCTAAATTTATACAGTTTCGATGTTTCT
TTTTCCGCCTAGAAGAGCTTCTCGGAAGCTAATGTCATGCCTCAAAATTAATTTGAAAAA
AAAAACTGAAAAAGGAAGGAAATTTGGTGAGAAGAAACATACAGTGGGAGGGTGAACAAA
GAATGGGCGCGACGCCGGAAAGGAAGACGACTGAGAAAGATACTCCGGTACCCGGAACGG
CGGAAAGCAAACAGATGACGAGCTGAATGGGCGGAGAAACCGGTGGCTTCGCCAGATCCG
TCGCGCGATCATTGTTATGAACGGTTGGTCTTTGGTAAAAAACAAAAAACAGTACAAGGG
AGAAGAAATAGACAAACAGATTGAGTTGATGGAAGATGTTTCTAAGAAGAGAGCTTAAAA
AGATAAATGTTACGTACCCGTGTGTTTGTTTCTGTTCGAGTATCAAACAGATGATAGTTT
AAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAAT
GAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTT
ACGTCAGTGGAGATT

Last Updated on 06.12.2018 16:11