Sequences from confirmation amplicon


Confirmation sequences for line 440E08 and hit At3g07160 are shown.
Line ID 440E08
Locus At3g07160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2265393
Primers used for PCR 40xe - 8474, show primer details
   
Sequence Name 48-K119006-0022-440-E08C-40xe
GenBank Accession LN506655 [GenBank]
Sequencing primer gene specific primer, 40xe: 5'-AAAATTATGCTTTCAGAGTCGTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K119006-0022-440-E08C-40xe
TAATTTTTCTAGCTTAGAAAATAATTATTATATCTGTGTGTCGACAGTCTCACATTCGGA
CCTTGAGCGGGAGGATAATGGAGACTATTTTAAGTCTACGATTCTTTATCTTCCAGTATG
GTATTGTCTACAAACTGAAGCTACAAGGATCAGATACATCATTTGCGGTAGAGCGCTCCC
CTTCATTGACTACTTGTATCTACGCCTTTGCATATCTTAGTCTGCTAGTATTTGTGCTTA
ATAATATCACTCATCTATCTGCAGGTGTACGGATGGTCATGGGTTGCATTTACAATGATT
ATAGTTCTTTTCAAGGTTAGTTTATTCCATCAGCATGCTTCATTTTCCGATAACCTTTGA
TCCTCTGACTAAATTTGATTACATGATCTGAGATATCTCCCATGTGAACTTTGAAATAGG
TCTTTACCTTCAGCCAGAAGATTTCTGTCAATTTCCAGCTTTTACTGAGGTTTATACAAG
GTCTATCCTTATTGATGGCTCTAGCTGGCATAATCGTAGCAGTTGTGCTCACACCCTTGT
CAGTGACAGACATATTTGCATGCGTATTGGCTTTTATACCGACAGGATGGGGAATTCTTT
CTGTAAGTTGTTCTTCTTGCTCACCTCAGATACCTTGGATTCTTTCTCATCTCTGCAGAT
TCCTCCTAATTTTGACAAACAAATCTTCTCCATA
   
Sequence Name 18-K119005-0022-440-E08C-8409
GenBank Accession LN494532 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K119005-0022-440-E08C-8409
GATTTGTTTGCTCAAATTAGGAGGAATCTGCAGAGATGAGAAAGAAATCAAGGTATCTGA
GGTGAGCAAGAAGAACTTCTTACAGAAAGAATTCCCCATCCTGTCGGTATAAAAGCCAAT
ACGCATGCAAATATGTCTGTCACTGACAAGGGTGTGAGCACAACTGCTACGATTATGCCA
GCTAGAGCCATCAATAAGGATAGACCTTGTATAAACCTCAGTAAAAGCTGGAAATTGACA
GAAATCTTCTGGCTGAAGGTAAAGACCTATTTCAAAGTTCACATGGGAGATATCTCAGAT
CATGTAATCAAATTTAGTCAGAGGATCAAAGGTTATCGGAAAATGAAGCATGCTGATGGA
ATAAACTAACCTTGAAAAGAACTATAATCATTGCAAATGCAACCCATGACCAACCGTACA
CCTGCAGATAGATGAGTGATATTATTAAGCACAAATACTAGCAGACTAAGATATGCAAAG
GCGTAGATACAAGTAGTCAATGAAGGGGAGCGCTCTACCGCAAATGATGTATCTGATCCT
TGTAGCTTCAGTTTGTAGACAATACCATACTGGAAGATAAAGAATCGTAGACTTAAAATA
GTCTCCATTATCCTCCCGCTCAAGGTCCGAATGTGAGACTGTCGACACACAGATATAATA
ATTATTTTCTAAGTTAGAAAAATTAGTTCTCAGACAAGCAAGAAAACTTTCTGAGAATAG
TTCTTACCAGTTCTTCTTCCCACCATGCTTCCCAGCTTTCAGCTCCTTTCACACCAATTC
CACCTCTGTAAAAGAGCCAATTTGTCCATTCTTGAAGTCCTCCACGACTCTGAA

Last Updated on 06.12.2018 16:11