Sequences from confirmation amplicon


Confirmation sequences for line 360A10 and hit At3g08010 are shown.
Line ID 360A10
Locus At3g08010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2556789
Primers used for PCR JR02 - 8474, show primer details
   
Sequence Name 11-K023017-022-360-A10C-8409
GenBank Accession LN490928 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K023017-022-360-A10C-8409
GACTTCCTTTTACACTGTGTACACGCGTCATCCCGGTTTCCAAAAGGGATCATTGCCTCT
TCTGTCTCTCGACAATCCCTTTCCAATGAATCTTCCGGAGAACTTGTTTGGGGAGAAATG
GGCATTTGTTCAATTACCTTACTCAGCTGTTAGAGAGGAGATCTCAGATTTTGATGAGAA
GTTTGTGTTTGGTGCTAGCTTAGACTTGGATTTACTTGGCATTGAAGTGGATGAGAATAC
TCTGATTCCTGGTCTCTCTGTTGCTACTTCACGTGCCAAACCTCTAGCAGGTAACTGTCC
GGTTTCTTGCTAGACCATCCTTTTGGANTTTTTTCTCCATTTTTTCCNAATTCTCATGAN
TTTTTCCATATATCTTTAACTACANTATGTTTGATCAAAAGTTGTTTCTTTTGTTCTTCC
TATTATGAATTCCTACAGAGATTTTAGGTTTGGTTCTTGTTGGTAATTATGTACCTTGGG
TTATCAAATATTGTAAGCTTT
   
Sequence Name 12-K023017-022-360-A10C-JR02
GenBank Accession LN491388 [GenBank]
Sequencing primer gene specific primer, JR02: 5'-CCAAAAGATGGTCTAGCAAGAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K023017-022-360-A10C-JR2
AGTTACCTGCTAGAGGTTTGGCACGTGAAGTAGCAACAGAGAGACCAGGAATCAGAGTAT
TCTCATCCACTTCAATGCCAAGTAAATCCAAGTCTAAGCTAGCACCAAACACAAACTTCT
CATCAAAATCTGAGATCTCCTCTCTAACAGCTGAGTAAGGTAATTGAACAAATGCCCATT
TCTCCCCAAACAAGTTCTCCGGAAGATTCATTGGAAAGGGATTGTCGAGAGACAGAAGAG
GCAATGATCCCTTTTGGAAACCGGGATGACGCGTGTACACAGTGTAAAAGGAAGTCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGAT
AAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAA
TTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11