Sequences from confirmation amplicon


Confirmation sequences for line 338G05 and hit At3g08840 are shown.
Line ID 338G05
Locus At3g08840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2684623
Primers used for PCR 09qk - 8474, show primer details
   
Sequence Name 67-K115459-0022-338-G05C-09qk
GenBank Accession LN511879 [GenBank]
Sequencing primer gene specific primer, 09qk: 5'-AGTACCAAACTACTTGGTGCAGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K115459-0022-338-G05C-09qk
CTCTTTTTGCATTCAATTCCCTCTTCATTGTATATTAAAATCTTATCTATACATGCACTC
AAATTTCGAGCTAGTGATATATTTCCTAAACACAAATCCTTGCTCAGGGAACTGGAGTAG
ACAAAAGTGAAATAGCACTGTGGTTTACAGATAACCAGCTGGATCTCGAGATGGGAAAAG
TTGTGGTAAGCATCTCCCCATTTCTCAGCCTGGTCATAGAAATGTTGCCTAGAGATCCCT
CCTCATTTATTTCACTTTTTTTTTTTCCCCCTCCACGTTGGTCTGTTTCAATTGAAAATG
GCTTCATGTCCGGGAAATCTACTTGGATCACCAATGAGTATGATGGTCAATATGGAAAAA
AAAAAAGAGTATTTCCCATTTTTTTTTCATTTCAAAAATGTAAATTTCCC
   
Sequence Name 18-K115458-0022-338-G05C-8409
GenBank Accession LN494436 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K115458-0022-338-G05C-8409
ACATTGAACAGACAAACGTGAAGAGAGAAAAAAAAAAAGTGAAATAAATGAGGAGGGATC
TCTAGGCAACATTTCTATGACCAGGCTGAAAAATGGGGAAATGCTTACCACAACTTTTCC
CATCTCGAGATCCAGCTGGTTATCTGTAAACCACAGTGCTATTTCACTTTTGTCTACCCC
AGTTCCCTGAGCAAGGATTTGTGTTTAGGAAATATATCACTAGCTCGAAATTTGAGTGCA
TGTATAGATAAAATTTTAATATACAATGAAAAGGGAATTGAATGCAATAAAAAAACATGC
CTGCATAGACAATAATACAATATACTACCTGCACCAAGTA

Last Updated on 06.12.2018 16:11