Sequences from confirmation amplicon


Confirmation sequences for line SALK_057821 and hit At3g08950 are shown.
Line ID SALK_057821
Locus At3g08950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2727707
Primers used for PCR 39fx - LBb3, show primer details
   
Sequence Name 62-K111812-SALK_057821C-39fx
GenBank Accession LN485793 [GenBank]
Sequencing primer gene specific primer, 39fx: 5'-TTGAGGTTGGGTCTTATTTTGTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K111812-SALK_057821C-39fx
TCCATTATCAATCAAGTATCGTTGGTCGCTCTTGAGTCAACTGGGTCTTCATGAAAAGAG
TTGGAACTAAGTGATAGTAATGTTAAAACTGGGGGTGGAAAGCATAAATATCTAAAACAA
TTGATTGAAACCGGCTTAATGGGCTTTAAATATCTAAAACTAATGAGCTTGTCGACCCAA
ACGGGCTTTAAACTGAACGGCAAGGTGTTTGTATAAAGGGCGTTCGCAAACCTTGCCGTT
TTAATATAAACTGTGTCGTTTCACTAGAAGGTTTTATAAAGTGTAAAACCTCTTTGCACC
TCTCAGATTAAAAACCTCAGCTCGCAGGAAAGAAATCAGCGAATTTGTGAACTTCAGAGA
AAAATTGGTCGTCAATGGCGTCTGCTCTATGTAGAACTGCAAGCCGTCTTCGTTCCGTTC
AACTTTTTCGTCGGATTCGCGTCTCCAGCGATCTACTATCGGCATCGTCTCCTTCTCCGG
CGTGCATCTCCGACGCGCTTCGTCATGGAGATTTCTCTCTTCCTCGATCGGTTCGTCATT
TCTTCTCCTCATCTGAATTCATTTTCTAATTCTCTTTGTTTTGAGCTCTCTAATTGCTAT
CTTCCTTTTGCAGTTTTTTTCACTTAACTGTGGAATCGAAATGCTGAAAATGGATCAGAG
ATGTTTGCTGAGTACTTCTGCTTCTGATACTACCTCGAAACATGACTCAGGTAAACCGGA
AACAAAATCTTCGGAGAAAAATGAGAAATCTGGAGGATCGGAGAGTTCTGATGGTGGTTC
AGATCATAAAAACGAGCGTGCTTCTGGAAAAGACGCTNAGACAACTTAATAACACATTGC
GGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGA
TTGCCCTTCACCGCCTGGCCCTGAGAAAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCC
CGCAGGCGAAAATCCTG
   
Sequence Name 52-K111811-SALK_057821C-R210
GenBank Accession LN485559 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K111811-SALK_057821C-R210
GCTCGTTTTATGATCTGAACACCATCAGAACTCTCCGATCCTCCAGATTTCTCATTTTTC
TCCGAAGATTTTGTTTCCGGTTTACCTGAGTCATGTTTCGAGGTAGTATCAGAAGCAGAA
GTACTCAGCAAACATCTCTGATCCATTTTCAGCATTTCGATTCCACAGTTAAGTGAAAAA
AACTGCAAAAGGAAGATAGCAATTAGAGAGCTCAAAACAAAGAGAATTAGAAAATGAATT
CAGATGAGGAGAAGAAATGACGAACCGATCGAGGAAGAGAGAAATCTCCATGACGAAGCG
CGTCGGAGATGCACGCCGGAGAAGGAGACGATGCCGATAGTAGATCGCTGGAGACGCGAA
TCCGACGAAAAAGTTGAACGGAACGAAGACGGCTTGCAGTTCTACATAGAGCAGACGCCA
TTGACGACCAATTTTTCTCTGAAGTTCACAAATTCGCTGATTTCTTTCCTGCGAGCTGAG
GTTTTTAATCTGAGAGGTGCAAAGAGGTTTTACACTTTATAAAACCTTCTAGTGAAACGA
CACAGTTTATATTAAAACGGCAAGGTTTGCGAACGCCCTTTATACAAACACCTTGCCGTT
CAGTTTAAAGCCCGTTTGGGTCGACAAGCTCATTAGTTTTAGATATTTAAAGCCCATTAA
GCCGGTTTCAATCAATTGTTTTAGATATTTATGCTTTCCACCCCCAGTTTTAACATTACT
ATCACTTAGTTCCAACTCTTTTCATGAAGACCCAGTTGACTCAAGAGCGACCAACGATAC
TTTGATTGATAATGGAGTAACCAAAGAACAATAAAAGAGTTTTGCATTAACTACCCCAAG
AGAACAAAAAAGAC

Last Updated on 06.12.2018 16:11