Sequences from confirmation amplicon


Confirmation sequences for line 044A02 and hit At3g12080 are shown.
Line ID 044A02
Locus At3g12080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3847999
Primers used for PCR HM07 - 8474, show primer details
   
Sequence Name 01-K028918-022-044-A02C-8409
GenBank Accession LN486442 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K028918-022-044-A02C-8409
ATATCCTGACAGGATGGGATAATCGAAGAAGAGGAAGATGGTGAAGAAGTCAATGAAGTT
GAAGAAGCTAGAGGAGAATAAATCCTAGAAATTGAGGGCATGGTGAAGAACCCTCTGGTT
GTCAACGAATCGAGTAGACTCGCCATTTTTGGCAGAGCAAAGAAAAAGAGAAGAAGAAGA
AGAAATTGCCAGAGAGGTTTATCACACTTATCTTCTGGGTTTTTAACTCTTTTATCGTTT
GATGGATCCGGAACCGGATGAACCGGTTTAATTCGCAAAACTCGATTCTTCTGGTTTTGA
TTAAAATCGGATTGGACATGAAGGATTTGAATCGGTTCAATTAAGAGAGCCAGATTGATT
TCGGTTT
   
Sequence Name 02-K028918-022-044-A02C-HM07
GenBank Accession LN486903 [GenBank]
Sequencing primer gene specific primer, HM07: 5'-GTAACCCAGACTAAACCGAAATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K028918-022-044-A02C-HM07
CTTCATGTCCAATCCGAATTTTAATCAAAACCAGAAGAATCGAGTTTTGCGAATTAAACC
GGTTCATCCGGTTCCGGATCCATCAAACGATAAAAGAGTTAAAAACCCAGAAGATAAGTG
TGATAAACCTCTCTGGCAATTTCTTCTTCTTCTTCTCTTTTTCTTTGCTCTGCCAAAAAT
GGCGAGTCTACTCGATTCGTTGACAACCAGAGGGTTCTTCACCATGCCCTCAATTTCTAG
GATTTATTCTCCTCTAGCTTCTTCAACTTCATTGACTTCTTCACCATCTTCCTCTTCTTC
GATTATCCCATCCTTGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTT
GATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTC
TAATTCGGAAATCTTTATTTCGACGTGTCTACATTC
Primers used for PCR CT40 - 3144, show primer details
   
Sequence Name 25-K105032-0022-044-A02C-CT40
GenBank Accession LN497435 [GenBank]
Sequencing primer gene specific primer, CT40: 5'-TCAAATGTTAGCAAAACTTACCCC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K105032-0022-044-A02C-CT40
TGATTTCCGACTTAGGCCTTCCACTATAGCAACTCTTTGGAGAAGATGCTCTGGTATCTG
CTGTGTCTGGTTTTTATTTTATTTTATAGTCATGTCAGTAATCTGAAGCCTCGTAGATAA
ACTAACAAAGAAAAAATTCAAAAATTCAATTGACTAACATTTTTGGCTAGCCTTTTCCCT
TTTCTACGAGTTTCCTTCCCCTCAATGGTCTCATCCTCTGGAAACATCAAACTATGATCA
CACACTGACAGTGTATTCCACACATATGCACAATAAAAACAGGGAAGAAATTACCGATTT
TGAGCTCGCGAGACAGAGTGGTAGCATAATCTCTCACGATATCTCTCGCTTCTTTCTCAA
GAACGGAGATATCGATGGAGTCGTCTTCGTCATCCGAATAATCATCTGCGAAGTTATCTT
CAGCATATTGGTCGAATTCCTCGAAATCTAGCTCTTCCTCAGCAGAGGAACCATCCAGAG
TTGCTGCAACAAGGAAAGGGAAGCGAGAGGAATGCGGGTGTTGGTGAGTGTAAGTCAAAC
ACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCT
CCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTG
CGTCATCCCTTACGTCAGTGGAGATAT
   
Sequence Name 41-K105033-0022-044-A02C-3144
GenBank Accession LN504075 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K105033-0022-044-A02C-3144
CCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACC
CGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGACTTACACTC
ACCAACACCCGCATTCCTCTCGCTTCCCTTTCCTTGTTGCAGCAACTCTGGATGGTTCCT
CTGCTGAGGAAGAGCTAGATTTCGAGGAATTCGACCAATATGCTGAAGATAACTTCGCAG
ATGATTATTCGGATGACGAAGACGACTCCATCGATATCTCCGTTCTTGAGAAAGAAGCGA
GAGATATCGTGAGAGATTATGCTACCACTCTGTCTCGCGAGCTCAAAATCGGTAATTTCT
TCCCTGTTTTTATTGTGCATATGTGTGGAATACACTGTCAGTGTGTGATCATAGTTTGAT
GTTTCCAGAGGATGAGACCATTGAGGGGAAGGAAACTCGTAGAAAAGGGAAAAGGCTAGC
CAAAAATGTTAGTCAATTGAATTTTTGAATTTTTTCTTTGTTAGTTTATCTACGAGGCTT
CAGATTACTGACATGACTATNAAATAAAATAAAAACCAGACACAGCAGATACCAGAGCAT
CTTCTCCAAAGAGTTGCTATAGTTGGAAGGCCTAATGTCGGAAAATCAGCATTGTTCAAC
CGCCTTGTTGGGGTAAGTTT

Last Updated on 06.12.2018 16:11