Sequences from confirmation amplicon


Confirmation sequences for line 908E11 and hit At3g12780 are shown.
Line ID 908E11
Locus At3g12780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4063301
Primers used for PCR 02be - 8474, show primer details
   
Sequence Name 42-K107369-0022-908-E11C-02be
GenBank Accession LN504482 [GenBank]
Sequencing primer gene specific primer, 02be: 5'-CTTCCCTGAAAGATCAAAGTGTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K107369-0022-908-E11C-02be
ACGTAGGAGTGTGTGGGAATATATTACCAGATGAGTGGAGAGGATAACTTTAGCACCATT
TTCAATCAAATACTTGATCGTTGGAATGGCGGCACGGATTCTGGTATCGTCAGTGATAGT
CTGATTATCATCGAGAGGTACATTGAGATCAGCTCTCACGAAAACCTTCTTCCCCTTCAA
ATCAGCTGAGGTCAGATCTCCGACGCTCTTCTTCGCCATAGAAACCACTCCTCTGCTTCC
CTTCCCACGCACCGATTCAACTTTGGAAGCAACGTGGAGAGAGAAACGACGGGAATCTAG
AGTGGCGGAGAAGCCTAGAGGACGCGCGGAAACAGAGGTAGAGGGAATTGGCAGAAGGGA
GGCACGTGCGCGAGTTCCGGCGGAGGAAGCAACAGCGCCGGTGGACTTAAGGAGTGAAAA
GGCGGAACTTGCGGCAGCGGAAGCCATGTGGATCAATCAAAGAAGATAAAAAGATGAGGA
GATGAGAAACAGAGAGAGTGACTCACAATGTTCTTATTTATTAGCCATTTGAGATTTTAT
GAGTTTGGAATATTTCTCTCTTATCACTGATGAGAGGTTCAAAAGCGATTATCTTTTTGA
TCATATATCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCG
   
Sequence Name 58-K107370-0022-908-E11C-8409
GenBank Accession LN509722 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K107370-0022-908-E11C-8409
GCCATTTACAATTGATATATGATCAAAAGATAATCGCTTTTGAACCTCTCATCAGTGATA
AGAGAGAAATATTCCAAACTCATAAAATCTCAAATGGCTAATAAATAAGAACATTGTGAG
TCACTCTCTCTGTTTCTCATCTCCTCATCTTTTTATCTTCTTTGATTGATCCACATGGCT
TCCGCTGCCGCAAGTTCCGCCTTTTCACTCCTTAAGTCCACCGGCGCTGTTGCTTCCTCC
GCCGGAACTCGCGCACGTGCCTCCCTTCTGCCAATTCCCTCTACCTCTGTTTCCGCGCGT
CCTCTAGGCTTCTCCGCCACTCTAGATTCCCGTCGTTTCTCTCTCCACGTTGCTTCCAAA
GTTGAATCGGTGCGTGGGAAGGGAAGCAGAGGAGTGGTTTCTATGGCGAAGAAGAGCGTC
GGAGATCTGACCTCAGCTGATTTGAAGGGGAAGAAGGTTTTCGTGAGAGCTGATCTCAAT
GTACCTCTCGATGATAATCAGACTATCACTGACGATACCAGAATCCGTGCCGCCATTCCA
ACGATCAAGTATTTGATTGAAAATGGTGCTAAAGTTATCCTCTCCACTCATCTGGTAATA
TTATTCCCACACACTCCTTACGTTTGATTCCTCCATCACCTGAACTAAAGTTGAACACTT
TGATCTTTCCAGGGAAG

Last Updated on 06.12.2018 16:11