Sequences from confirmation amplicon


Confirmation sequences for line 549C05 and hit At3g13724 are shown.
Line ID 549C05
Locus At3g13724 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4496874
Primers used for PCR NK26 - 8474, show primer details
   
Sequence Name 47-K102592-0022-549-C05C-8409
GenBank Accession LN506165 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K102592-0022-549-C05C-8409
TCGGAATGACCATTTACATTAATGAGTATATCACCATTTAGTTCTAGTGGGTGGCGTTAA
TATAAGATTGGTAATGGTAACAGTGAAACATATCCCCCATCTATTGGGATTAATTAGTCT
CCCTGAACAGTCGTGAGAGAACCTTTGTTTGCTGCGGCAGACACTAGAGACTGTTGGAAG
ACCTTGAGATATACAAAAATGTAGAATTATTTTGAAAATAGGTGATGTGGATCTGGTACT
ACTTGATTTACATATATGTATTTGTTTCTCTGGTTCTTATGGAGAAAAACTTAAGATTTT
TGTAATCCATCATACATTTTGGTATAGTTTTGATCTTATATGTATTGTGCTTTGACACAA
GCTAACTATTTTCTTGTTTCACAGAGCGGGATCAATTCTAAAGCAGCCATGAGTATGATG
GTCAATAT
   
Sequence Name 47-K102593-0022-549-C05C-NK26
GenBank Accession LN506166 [GenBank]
Sequencing primer gene specific primer, NK26: 5'-ACTCATGGCTGCTTTAGAATTGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K102593-0022-549-C05C-NK26
TAGCTTGTGTCAAGCACAATACATATAAGATCAAAACTATACCAAAATGTATGATGGATT
ACAAAAATCTTAAGTTTTTCTCCATAAGAACCAGAGAAACAAATACATATATGTAAATCA
AGTAGTACCAGATCCACATCACCTATTTTCAAAATAATTCTACATTTTTGTATATCTCAA
GGTCTTCCAACAGTCTCTAGTGTCTGCCGCAGCAAACAAAGGTTCTCTCACGACTGTTCA
GGGAGACTAATTAATCCCAATAGATGGGGGATATGTTTCACTGTTACCATTACCAATCTT
ATATGATCACCACCCACTAGAACTAAATGGTGATATACTCATTAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAA
TGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAA
TAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCAC

Last Updated on 06.12.2018 16:11