Sequences from confirmation amplicon


Confirmation sequences for line 508H09 and hit At3g15830 are shown.
Line ID 508H09
Locus At3g15830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5355601
Primers used for PCR TH23 - 8474, show primer details
   
Sequence Name 50-K103582-0022-508-H09C-TH23
GenBank Accession LN507138 [GenBank]
Sequencing primer gene specific primer, TH23: 5'-TCGCCAAATAGGACCAATAAATAG-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K103582-0022-508-H09C-TH23
CGGTGGAGGAGGAGAATGGACAAGCAAAGCGTCGTTTATGACGTGGACGATGCATGACAT
AATTTACGTGGCGAGACACCATTGGATACCGTGTTTATTCGCGGCCGGAGTTATGTTTTT
TACGGTTGTGGAGTACACGTTCCAGATGACTCCTGCGAGTTCTCAGCCGTTTGATCTAGG
ATTTGTGGCCACACGTTATCTGCATAGCATCTTGGCATCTTCACCCAATCTTAACACCGT
TTTAGCCGCTTTAAACACGGTAAACACATCTTTTATGACACTATCCTCAAATTCCTCGCA
TTATGATATTCACATGCAACAAAGAGAGACTAGTTAAATTCGACTTAATTTTGCATTTTT
TTTAATCAATTGTTTTGGTTGGGGGTGTGGACAGATTTTGGTAGGGATGCAAACAACGTA
TATTGGATGTACATGGGCAGTGGAAGGACGACCACGGGCAAATGTCGACACGTATATNTT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
C
   
Sequence Name 66-K103583-0022-508-H09C-8409
GenBank Accession LN511587 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K103583-0022-508-H09C-8409
TAAATTGAATATATACGTGTCGACATTTGCCCGTGGTCGTCCTTCCACTGCCCATGTACA
TCCAATATACGTTGTTTGCATCCCTACCAAAATCTGTCCACACCCCCAACCAAAACAATT
GATTAAAAAAAATGCAAAATTAAGTCGAATTTAACTAGTCTCTCTTTGTTGCATGTGAAT
ATCATAATGCGAGGAATTTGAGGATAGTGTCATAAAAGATGTGTTTACCGTGTTTAAAGC
GGCTAAAACGGTGTTAAGATTGGGTGAAGATGCCAAGATGCTATGCAGATAACGTGTGGC
CACAAATCCTAGATCAAACGGCTGAGAACTCGCAGGAGTCATCTGGAACGTGTACTCCAC
AACCGTAAAAAACATAACTCCGGCCGCGAATAAACACGGTATCCAATGGTGTCTCGCCAC
GTAAATTATGTCATGCATCGTCCACGTCATAAACGACGCTTTGCTTGTCCATTCTCCTCC
TCCACCGTCATCAGTTTTCTGAGCAATATCTTCCATTTGGCTATTTATTGG

Last Updated on 06.12.2018 16:11