Sequences from confirmation amplicon


Confirmation sequences for line 901E08 and hit At3g16640 are shown.
Line ID 901E08
Locus At3g16640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5670696
Primers used for PCR MN43 - 8474, show primer details
   
Sequence Name 01-K032412-022-901-E08C-8409
GenBank Accession LN486458 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K032412-022-901-E08C-8409
CGGATGAGCCTTTCATTGATCCTGTCCGGACATCATACTCTTTCTTTTTCACAGTTCATT
GCTTACCGGTGAGAAGATCTTGGTACACCAACATGGTCGCTTATTGATTGTTTTCTCTCT
CCGATGGATGCGAATAAACGAAAATCGAAGGTTGCGGGAGAGAGACGCAGCGACGAGGGG
ATTCTGAAGTGAGGTCCTAAGTTTGGTATTTATAGTCGAAACTATAGCGTAGGGTTTTCA
GGTAAATTTACAATTCTCGGTAAATTACAATTTTGGGCTTTAACTGTTTCCAATTTTCTA
TTTTTTACCCTTTCCTTTTTTTTAAATAAAGGAAATAAATCTGAACCGACGCTTAATGAG
CCGGGTTTGGTCCAACGGGTGGGGATTCGAGTGTTGGGCTACGTATAAATAGTGTTCGGG
TCGGATAAAATCCTATTTCCCTCTCTTCTCTTCCGTCTTTCTCGTTCTGTTTCTGATTGT
TCGTGTAAAACCCTAGAATGACGATG
   
Sequence Name 01-K032412-022-901-E08C-MN43
GenBank Accession LN486459 [GenBank]
Sequencing primer gene specific primer, MN43: 5'-TGTTCAAAGCTATCATCGTCATTC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K032412-022-901-E08C-MN43
CAGAACGAGAAGACGGAAGAGAAGAGAGGGAAATAGGATTTTATCCGACCCGAACACTAT
TTATACGTAGCCCAACACTCGAATCCCCACCCGTTGGACCAAACCCGGCTCATTAAGCGT
CGGTTCAGATTTATTTCCTTTATTTAAAAAAAAGGAAAGGGTAAAAAATAGAAAATTGGA
AACAGTTAAAGCCCAAAATTGTAATTTACCGAGAATTGTAAATTTACCTGAAAACCCTAC
GCTATAGTTTCGACTATAAATACCAAACTTAGGACCTCACTTCAGAATCCCCTCGTCGCT
GCGTCTCTCTCCCGCAACCTTCGATTTTCGTTTATTCGCATCCATCGGAGAGAGAAAACA
ATCAATAAGCGACCATGTTGGTGTACCAAGATCTTCTCACCGGTAAGCAATGAACTGTGA
AAAAGAAAGAGTATGATGTCCGGACATGGATCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACAT
TTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTA
TTCTAATTCGGAAATCTTTATTTCGACGTGTCTACT

Last Updated on 06.12.2018 16:11