Sequences from confirmation amplicon


Confirmation sequences for line 162B10 and hit At3g18350 are shown.
Line ID 162B10
Locus At3g18350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6299835
Primers used for PCR 02us - 8474, show primer details
   
Sequence Name 63-K118152-0022-162-B10C-02us
GenBank Accession LN511082 [GenBank]
Sequencing primer gene specific primer, 02us: 5'-CACTCTCTCAACCGCCTTATTAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K118152-0022-162-B10C-02us
TGAAAGCTCAACAATACAACCGAAAGCAAGTAGCGTCCCGGAACAAAGGCCATCATCGTC
GCTGCGACCACAATCGCAATCGCAAACTTCTGGCTCGCCTGAAATAGCGGTTTCAAAACT
GTGGTTAGACACAGCTTTAGGAGAAGCAGTTTGTGTTAAATATAAGAGGGAAGGCTGACC
TGAGGGAAAAGCGAGAATAATAAAGCTCGGAACTTGAGAAGAACGATGTTTGCGTCTTGA
ATGTTCTGTTCGAGCTCCGAGATCCCGTTCTGGACTGCCAAAAGCTGTTCCATTGTGTTC
ATTGGAGGAGGAGCAGTTACTTTCAGCTCGATCATGAGCTTTTCTCTGCTGAAACATCTT
GTGAGAACCATGAAGATGGCAGAGAAGAGAGACGCAAGAGCAAAGACATAAACAAGCCAT
CCCCTCCAAATTATGAAAGTGGTCAAGAGACAGAAAACACTAGACTTTGTAGGATCATCC
CAATACACCACAGATAGAATTAAGTTCCAGGTTTCCATTATTGGAAGCATCAGGATATAT
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCACGGTTTATTA
   
Sequence Name 15-K118151-0022-162-B10C-8409
GenBank Accession LN493171 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K118151-0022-162-B10C-8409
TCATTGAATATATCCTGATGCTTCCATAATGGAAACCTGGAACTTAATTCTATCTGTGGT
GTATTGGGATGATCCTACAAAGTCTAGTGTTTTCTGTCTCTTGACCACTTTCATAATTTG
GAGGGGATGGCTTGTTTATGTCTTTGCTCTTGCGTCTCTCTTCTCTGCCATCTTCATGGT
TCTCACAAGATGTTTCAGCAGAGAAAAGCTCATGATCGAGCTGAAAGTAACTGCTCCTCC
TCCAATGAACACAATGGAACAGCTTTTGGCAGTCCAGAACGGGATCTCGGAGCTCGAACA
GAACATTCAAGACGCAAACATCGTTCTTCTCAAGTTCCGAGCTTTATTATTCTCGCTTTT
CCCTCAGGTCAGCCTTCCCTCTTATATTTAACACAAACTGCTTCTCCTAAAGCTGTGTCT
AACCACAGTTTTGAAACCGCTATTTCAGGCGAGCCAGAAGTTTGCGATTGCGATTGTGGT
CGCAGCGACGATGATGGCCTTTGTTCCGGGACGCTACTTGCTTTCGGTTGTATTTGTTGA
GCTTTTCACAAGATACTCGCCACCAAGGAGAGCTAGCACGGAACGGTTAATAAGGCGGTG
AAGAGAGTG

Last Updated on 06.12.2018 16:11