Sequences from confirmation amplicon


Confirmation sequences for line 170A04 and hit At3g18930 are shown.
Line ID 170A04
Locus At3g18930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6523956
Primers used for PCR AC38 - 8474, show primer details
   
Sequence Name 49-K027911-022-170-A04C-8409
GenBank Accession LN506715 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K027911-022-170-A04C-8409
CATTTACATTGAATGTGAGCAGCTGAGCAATTTCTGAATCTCTCCATCTTCTTCTGTTTC
GTTCATTTTAATTCCATTAAATGCTTAAACCCCACATTCCATTAATGCTCTCTCCTTCCA
CTTCAATATTTCTTTTTATCATCACCACCGTCCAAATTTCATCTTACATCTCTGCCCTAC
ACGTGTCTCTTCTTCTCCATCTCTCCGGCGATAACAGTGCCTCTGGATCTCCGCATCTTA
ACCGGCTCGATGAGTGCGGTGCTGTTCTGAAGAACATCGGACTCGTCATCGATTTCGTTC
GCCGCGATGAGCTTCCCGTCGCTGGAATCCGTTCTGATATCGGGTAAAACACTCCCGCCG
ATGATCTCCGTCGGAAGAACGGTGATTTCGTGCTTCTGTAGTAACGGAACGAGAACACTC
TTGATTTCGATATTAGGTTTCCGAACGGTGATTGACGTTTGTTCCATGTAGATCTCCGGT
ATCTCCATGGAGACACCGTCGCTGGCTCCATCGTTACTCTCTCTGATTCAGATTCTCTCT
CGCATTCGAACGAGTACGATCTCTTCAGCTCCCGGAACCGAGAAATCGCCGGTGATGATT
GCTCTTCCACCGACGCTCTGATCTCCTGATCGTTCGTCGTATCGGCAGCGATTGTGTTCC
AATTGCTCCGCGACGGTGTGATTTCGCCGCGGATGATGATCGCGTTATTCTC
   
Sequence Name 50-K027911-022-170-A04C-AC38
GenBank Accession LN507053 [GenBank]
Sequencing primer gene specific primer, AC38: 5'-CGAGTCTCGATGATGAAGAGAATA-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K027911-022-170-A04C-AC38
TCGCGGAGCATTGGAACCAATCGCTGCCGATACGACGAACGCTCTGGAGATCAGAGCGTC
GGTGGAAGAGCAATCATCACCGGCGATTTCTCGGTTCCGGGAGCTGAAGAGATCGTACTC
GTTCGAATGCGAGAGAGAATCTGAATCAGAGAGAGTAACGATGGAGCCAGCGACGGTGTC
TCCATGGAGATACCGGAGATCTACATGGAACAAACGTCAATCACCGTTCGGAAACCTAAT
ATCGAAATCAAGAGTGTTCTCGTTCCGTTACTACAGAAGCACGAAATCACCGTTCTTCCG
ACGGAGATCATCGGCGGGAGTGTTTTACCCGATATCAGAACGGATTCCAGCGACGGGAAG
CTCATCGCGGCGAACGAAATCGATGACGAGTCCGATGTTCTTCAGAACAGCACCGCACTC
ATCGAGCCGGTTAAGATGCGGAGATCCAGAGGCACTGTTATCGCCGGAGAGATGGAGAAG
AAGAGACACGTGTAGGGCAGAGATGTAAGATGAAATTTGGACGGTGGTGATGATAAAAAG
AAATATTGAAGTGGAAGGAGAGAGCATTAATGGAATGTGGGGTTTAAGCATTTAATGGAA
TTAAAATGAACGAAACAGAAGAAGATAGAGAGATTCAGAAATTGCTCAGCTGCTCACATT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGT
ATTTATAGGCGAAAGCAATAAACAAATTATTTCTATTCGGAAATC

Last Updated on 06.12.2018 16:11