Sequences from confirmation amplicon


Confirmation sequences for line 163D11 and hit At3g19710 are shown.
Line ID 163D11
Locus At3g19710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6849070
Primers used for PCR ML06 - 8474, show primer details
   
Sequence Name 49-K030753-022-163-D11C-ML06
GenBank Accession LN506729 [GenBank]
Sequencing primer gene specific primer, ML06: 5'-ATAAACCCTAATCATTCCCAAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K030753-022-163-D11C-ML06
TTAGTATCAAACCATTACTTAAGAATTGTTGCGAAACTAGATCACATACAATGATTTGAT
AGGAATATCAATACTTTCATACTATTCGGTTCGTTGATATACATTAATACATATATGTAC
AACTATTTCATTTTTTCAATAGTTTATAGCTCAAATATGCATACAATATAGTTGCTAACT
CAATAGTATACTATGTAATTATATTTATGATAATTAGCTCAATCATTTTATTATCAGTGA
GAAAGCTATATATTCATGTGTTAATAGTTTTTTCTGTTTCATCTAATAACGAATATATTC
GAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACG
ATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATT
TCGACGTGTCTACATTCA
   
Sequence Name 10-K030753-022-163-D11C-8409
GenBank Accession LN490529 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K030753-022-163-D11C-8409
CCATTTACATTGAATATATTCGAATATATTCGTTATTAGATGAAACAGAAAAAACTATTA
ACACATGAATATATAGCTTTCTCACTGATAATAAAATGATTGAGCTAATTATCATAAATA
TAATTACATAGTATACTATTGAGTTAGCAACTATATTGTATGCATATTTGAGCTATAAAC
TATTGAAAAAATGAAATAGTTGTACATATATGTATTAATGTATATCAACGAACCGAATAG
TATGAAAGTATTGATATTCCTATCAAATCATTGTATGTGATCTAGTTTCGCAACAATTCT
TAAGTAATGGTTTGATACTAATTTACTGGAATATTTATGGTGGTTTGGGAATGATAGGGG
TTT

Last Updated on 06.12.2018 16:11