Sequences from confirmation amplicon


Confirmation sequences for line 532F01 and hit At3g20740 are shown.
Line ID 532F01
Locus At3g20740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7249946
Primers used for PCR AW06 - 8474, show primer details
   
Sequence Name 19-K104847-0022-532-F01C-8409
GenBank Accession LN494751 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K104847-0022-532-F01C-8409
TGGAGCTGTAATACTACATATAGAAGTAATGTTATTTACAAAGGCCCTCACCAATATTTG
TATGTCACTTGTTGTTACAAGACAAGGCTAAAACAAAATACTTACAGGGAATTGGACAAA
TTTTGTGGGGAATTTTGATGGATCATCAGTCCATGTGAATGACTTCTCGACGTACGTCCA
AAACTCTATAGACAGCCAAAGAATAAGAAGATCATACAAACGAACCACAAGCCATTTTTC
TCAAAAGGTTTTTTAAAATGGAAGTTTATTACAATATGTGCTCGATCGTACCTTTCATTG
ACCATATTTTAATAGTGGTGTCCATACCACAACTAGCAAAGCGGTAAATATCAGACGGAT
GAAAATCCTACAATTTTCAAAATAAGATGGTTCCCCTTAACATTGTCTCTATATATTATT
GTAGAAAACTAACTGAATTAGATAAAACAATATTGGCTCACCACACTTAGAACT
   
Sequence Name 04-K104846-0022-532-F01C-AW06
GenBank Accession LN487908 [GenBank]
Sequencing primer gene specific primer, AW06: 5'-AAGTTCTAAGTGTGGTGAGCCAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K104846-0022-532-F01C-AW06
ATAGAGACAATGTTAAGGGGAACCATCTTATTTTGACATTGTAGGATTTTCATCCGTCTG
ATATTTACCGCTTTGCTAGTTGTGGTATGGACACCACTATTAAAATATGGTCAATGAAAG
GTACGATCGAGCACATATTGTAATAAACTTCCATTTTAAAAAACCTTTTGAGAAAAATGG
CTTGTGGTTCGTTTGTATGATCTTCTTATTCTTTGGCTGTCTATAGAGTTTTGGACGTAC
GTCGAGAAGTCATTCACATGGACTGATGATCCATCAAAATTCCCCACAAAATTTGTCCAA
TTCCCTGTAAGTATTTTGTTTTAGCCTTGTCTTGTAACAACAAGTGACATACAAATATTG
GTGATGGCCTTTGTAAATAACATTACTTCTATATGTAGGTATTTACAGCTTCCATTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT

Last Updated on 06.12.2018 16:11