Sequences from confirmation amplicon


Confirmation sequences for line 008E03 and hit At3g21720 are shown.
Line ID 008E03
Locus At3g21720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7652884
Primers used for PCR BG76 - 8474, show primer details
   
Sequence Name 04-K104021-0022-008-E03C-BG76
GenBank Accession LN487892 [GenBank]
Sequencing primer gene specific primer, BG76: 5'-AATGGTCCGGTGCTAATTACTATG-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K104021-0022-008-E03C-BG76
GATCTCCTCACTGCAGCCATGGGAAAAGGTATGTTAGTTTATGTTTTTAACATTTATTTG
CTCACATAAAGTAACTTTAGTGAAATAATAGTGTGTTATGATAAGATTCTGCTTATAAGA
ACAATGAAAAGGAGTTTATATGTGACGTTCTTTATATCTTTCAACTATAATTGTTTGGTT
ACTAAAGATTCGTCCGGTTGGTCGAGACAAGTAATAGAGTCATTTCTATTACACTTCTAT
TATAGGTAATGTAGAAAATATAAATGCATTAAGTAGTACGTAGTGAAAAAACCCGGTAGA
AGTAATCCAATTATAGAATATTTTTTCTTTCAAAATTAACAATGACGTTACCCTTTTTCA
TATATAGGTGATGTCCGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTTT
   
Sequence Name 20-K104022-0022-008-E03C-8409
GenBank Accession LN495185 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K104022-0022-008-E03C-8409
GCCATTTACATTGAATATATCCTGCGGACATCACCTATATATGAAAAAGGGTAACGTCAT
TGTTAATTTTGAAAGAAAAAATATTCTATAATTGGATTACTTCTACCGGGTTTTTTCACT
ACGTACTACTTAATGCATTTATATTTTCTACATTACCTATAATAGAAGTGTAATAGAAAT
GACTCTATTACTTGTCTCGACCAACCGGACGAATCTTTAGTAACCAAACAATTATAGTTG
AAAGATATAAAGAACGTCACATATAAACTCCTTTTCATTGTTCTTATAAGCAGAATCTTA
TCATAACACACTATTATTTCACTAAAGTTACTTTATGTGAGCAAATAAATGTTAAAAACA
TAAACTAACATACCTTTTCCCATGGCTGCAGTGGAGGAGATTCCACCTTGGACGGTCTTA
AGATAACGATCATAGTAATTAGCACGGGACCA

Last Updated on 06.12.2018 16:11