Sequences from confirmation amplicon


Confirmation sequences for line 821C03 and hit At3g22231 are shown.
Line ID 821C03
Locus At3g22231 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7846591
Primers used for PCR CS26 - 8474, show primer details
   
Sequence Name 74-K031321-022-821-C03C-8409
GenBank Accession LN512995 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K031321-022-821-C03C-8409
TTACAATTGAATATATCCTGGACATGTAGATTTGTGAAGAGGAGAGAGTGTTGTGCTTCT
AATTTATAAGGTAGACAAATATTAGGAAATTTTTTACATTATTCATTATATAAAAGAAAG
AAGAGTCAATATTGAACAATAAAAACATCTAATCACAGACAACTCACGGACAGACCTACC
TTAGCGAAGTGGTAGGTCTAGGTTTTTTGGTTATATCCAACACATATATAATTGCAGAGA
TAAAGCAAATGATATTGTTTGAAATGCACAAGGGCAGAGGAAAGCGATACCTATGGTTGA
GATGAAAGTGAGAAATGGTTTGTTGCAGAAATGGCTTTCGTCACGTACATGACGTACACG
TACATGACGTCGTAAAACATGAGGAATTTAACAAATTTGAATTATTTTGTTCCTGGAACT
GTCTTTTAGAACCTCTTTTTTTTTTTTTGGGGTATGGCCCAAAACCCTAGGAAAATAAAA
AAACCCCTTTGGAGGATAAATTAATTTAACTTTTCGGGAAAAGGAAAAAGTGTTTACCCA
C
   
Sequence Name 75-K031321-022-821-C03C-CS26
GenBank Accession LN513168 [GenBank]
Sequencing primer gene specific primer, CS26: 5'-CAAAGGCAGTTGGTTAATCACTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K031321-022-821-C03C-CS26
TATTATTATCATCAAAAGAGGTTATTTAATTTACATAGAGTTTAGAGCAATACCCAAAAA
AAAAAAAAAGGGGTTCAAAAAAACGTTCCCGGGAACAAAAAAATTCAAATTTGTAAAATC
CCCCAGGTTTAACAA

Last Updated on 06.12.2018 16:11