Sequences from confirmation amplicon


Confirmation sequences for line 354E10 and hit At3g24200 are shown.
Line ID 354E10
Locus At3g24200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8748250
Primers used for PCR 57yx - 8474, show primer details
   
Sequence Name 30-K123352-0022-354-E10C-57yx
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 57yx: 5'-ATTTGTACTGTTGAGCATACAGCC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K123352-0022-354-E10C-57yx
GCGACCATATCTGCCAAAGAGAGGTTTGAAACTCCTCCCAAGGTTGTGAAGCTTTCTTCA
GAGAGAATGATGTTCCCTTTGTCTTTAAGGCACGCAAAAGACTATGTGTCAAAACGTGTG
GCTCTTGTTGGTGACTCAGCTCATACAGTTCACCCGTTGGCTGGTCAAGGAGTAAATCTA
GGATTTGCAGATGCGTGTGCTCTCACCAAAGCTATAGCAGAGGGCATTGCTCTTGGTACT
GATATAGGCGAGGTATGTGCTCGGATTCTAAGTCTTATACCTCATGTTTATTTTCTCCTT
TCCTCTGTATTGAATATTGATTGCTTGAAATTTTAAATTGTAGGCGAATCTGCTCAAACG
TTATGAAGCAGAGAGAAAACCTGCAAATATAGCAATGATGGCGGATTCCAACACTATGCA
CTCGTTGAAAAAGAAAAACCAGATACAAATATACAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACCGTTA
   
Sequence Name 14-K123351-0022-354-E10C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K123351-0022-354-E10C-8409
CATTTACATTGTATATTTGTATCTGGTTTTTCTTTTTCACGAGTGCACAGTGTTGGAATC
CGCCATCATTGCTATATTTGCAGGTTTTCTCTCTGCTTCATAACGTTTGAGCAGATTCGC
CTACAATTTAAAATTTCAAGCAATCAATATTCAATACAGAGGAAAGGAGAAAATAAACAT
GAGGTATAAGACTTAGAATCCGAGCACATACCTCGCCTATATCAGTACCAAGAGCAATGC
CCTCTGCTATAGCTTTGGAGAGAGCACACGCATCTGCAAATCCTAGATTTACTCCTTGAC
CAGCCAACGGGTGAACTGTATGAGCTGAGTCACCAACAAGAGCCACACGTTTTGACACAT
AGTCTTTTGCGTGCCTTAAAGACAAAGGGAACATCATTCTCTCTGAAGAAAGCTTCACAA
CCTTGGGAGGAGTTTCAAACCTCTCTTTGGCAGATATGGTCGCATCTCCTGTAAGCCAAG
AGAGACTATCCCGTGAACTTGTAGTCTCTGGATTAGGACCATATCCAGAATCCAGAGCAT
CATTTACAGCTTTGATGAAGTCATCCTCGTTCATCAATTTTCGATCTGATGCCTCTTTTG
GGTCCATGGTCCATACTATGTTGCTAAACTTGTCACCAATGGGAAGAAGTGCGATTGGGC
CATTGGGTAAAAACCGTTGCCATGCTGTGTAGTTTTCGGCTGTATGCTCAACAGTACAAA
T

Last Updated on 06.12.2018 16:11