Sequences from confirmation amplicon


Confirmation sequences for line 522B11 and hit At3g26910 are shown.
Line ID 522B11
Locus At3g26910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9915250
Primers used for PCR 07hd - 8474, show primer details
   
Sequence Name 46-K112689-0022-522-B11C-07hd
GenBank Accession LN505912 [GenBank]
Sequencing primer gene specific primer, 07hd: 5'-GATATAGTAGCATCCCCACCGTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K112689-0022-522-B11C-07hd
TGCTCAGACCAGTCAAATCAGAGGTATAATCGATTCTTTCAAACTCATTTTATGCGTTTA
CGTGAAATAATCTTACAGAAAAAGAATGATATTTTTGAAACATAAAAGCATGTTCAGTTT
TCCATAAAACACAAATGAAATGTTCTTGTTTAACTTTTGTTTGCGTTTGGCGTTTAGAAT
GACACTTTCAATCTACACGCAAACGCAAACGACTCGAAAATGTAACTGCATGAAGTATTT
TTAAAATGTAATTGCATGATTCATAAATGGAATTTGTTTGCTGTGGATTTATTGAAACCT
CCATAGGTTATTAATCATGCAGGAGTAGGAAAGCTGATCGAACGATGAGGCTTCCGCGTC
TGCGTCACGTTTGACCAAAACGCTGATTGTACAAATTCAGAGTCCAATTCACGCACGCAA
TTCTGATGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATTCC
   
Sequence Name 14-K112688-0022-522-B11C-8409
GenBank Accession LN492578 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K112688-0022-522-B11C-8409
TTACATTGAATATATCCTGACATCAGAATTGCGTGCGTGAATTGGACTCTGAATTTGTAC
AATCAGCGTTTTGGTCAAACGTGACGCAGACGCGGAAGCCTCATCGTTCGATCAGCTTTC
CTACTCCTGCATGATTAATAACCTATGGAGGTTTCAATAAATCCACAGCAAACAAATTCC
ATTTATGAATCATGCAATTACATTTTAAAAATACTTCATGCAGTTACATTTTCGAGTCGT
TTGCGTTTGCGTGTAGATTGAAAGTGTCATTCTAAACGCCAAACGCAAACAAAAGTTAAA
CAAGAACATTTCATTTGTGTTTTATGGAAAACTGAACATGCTTTTATGTTTCAAAAATAT
CATTCTTTTTCTGTAAGATTATTTCACGTAAACGCATAAAATGAGTTTGAAAGAATCGAT
TATACCTCTGATTTGACTGGTCTGAGCAACTCCTGTGGCTTGAGGAAGTGGCCTAGACAG
GCTCATTGGTGTTAACGGTGGG

Last Updated on 06.12.2018 16:11