Sequences from confirmation amplicon


Confirmation sequences for line 052G05 and hit At3g29725 are shown.
Line ID 052G05
Locus At3g29725 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:11556063
Primers used for PCR 42mv - 8474, show primer details
   
Sequence Name 72-K114263-0022-052-G05C-42mv
GenBank Accession LN512770 [GenBank]
Sequencing primer gene specific primer, 42mv: 5'-GACTCAACCACAGTGAAGAGACAG-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K114263-0022-052-G05C-42mv
ATCTTGTGGATCCCATTTGATGTGACCGAGAGTGGGAGGAAGTTAGTGAGGGCAAGGGAG
AGAGATTGCTCGAGCTTTGGGAGGATTGTGGAGAAGAAAGAGTCGTGAGATGATGACTCA
GAGAGTTTGTAAAAGGTGACTCGCTCGATGGGATGGTGTTTTAGCCAAAGTAAATCGAAG
AAAGTGAGTGGGAGGAAGAGCGGGGTGACTGAGTTAGTTGCATGGCTGACTCGGGAGATC
TTGATCATGTTAAGAGCCATCGTTGATGGAGACAATTTCACTTGGACTCAATTTCAGGTA
TTTGTTTCAAAACAAAATTTCACCTTAGCCTTCTATCTCAGTTTTTCTTTTAGATTTCAG
GATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGAAGTCCGCACGNTTATT
   
Sequence Name 29-K114262-0022-052-G05C-8409
GenBank Accession LN499391 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K114262-0022-052-G05C-8409
ATTTCATTGAATATATCCTGAAATCAAAAGAAAAACTGAGATAGAAGGCTAAGGTGAAAT
TTTGTTTTGAAACAAATACCTGAAATTGAGTCCAAGTGAAATTGTCTCCATCAACGATGG
CTCTTAACATGATCAAGATCTCCCGAGTCAGCCATGCAACTAACTCAGTCACCCCGCTCT
TCCTCCCACTCACTTTCTTCGATTTACTTTGGCTAAAACACCATCCCATCGAGCGAGTCA
CCTTTTACAAACTCTCTGAGTCATCATCTCACGACTCTTTCTTCTCCACAATCCTCCCAA
AGCTCGAGCAATCTCTCTCCCTTGCCCTCACTAACTTCCTCCCACTCTCTGGTCACATCA
AATGGGATCCACAAGATCCTAAGCCACATGTCATCATCTTCCCACAAGACACTGTCTCTT
CACTGGGGTTGAGT

Last Updated on 06.12.2018 16:11