Sequences from confirmation amplicon


Confirmation sequences for line 701G02 and hit At3g30300 are shown.
Line ID 701G02
Locus At3g30300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:11924026
Primers used for PCR PH20 - 8474, show primer details
   
Sequence Name 63-K117751-0022-701-G02C-PH20
GenBank Accession LN511075 [GenBank]
Sequencing primer gene specific primer, PH20: 5'-CTCCATTAGCAAAAGAACCACTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K117751-0022-701-G02C-PH20
GTCTTCTTGGTCTTGTTCTCTCTGCTTTCTCTCTTCTTGTCCATTTTCTTCTCGCTGGAT
TCACAGATGACTCAATCTCCGATTACTCTATCCCTGTCACTATCTTCTCTTGGAGACCTA
TCTTCGACAATCCCAGATTTGCCCGTCATGTAAAAATCTCACCTTTCTCTTTTGTTTAGT
GGATCTTGAAATTTTATTTTATTTCATCAAGTGTAATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGC
   
Sequence Name 38-K117750-0022-701-G02C-oriL
GenBank Accession LN503136 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K117750-0022-701-G02C-oriL
ACTAAACAAAGTGAAAGGGGAGATTTTTACATGACGGGCAAATCTGGGATTGTCGAAGAT
AGGTCTCCAAGAGAAGATAGTGACAGGGATAGAGTAATCGGAGATTGAGTCATCTGTGAA
TCCAGCGAGAAGAAAATGGACAAGAAGAGAGAAAGCAGAGAGAACAAGACCAAGAAGACC
AATCCATTTCATACGCGACTTCAAGAAGACTTTACCTTCTCCCTTCATCTCTACTTCAAG
ATGTGAAAGTGAAGTGAAGTGAAGTGGTTCTTTGCCTAATGGAG

Last Updated on 06.12.2018 16:11