Sequences from confirmation amplicon


Confirmation sequences for line 457F03 and hit At3g43970 are shown.
Line ID 457F03
Locus At3g43970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:15777782
Primers used for PCR 32gp - 8474, show primer details
   
Sequence Name 31-K112248-0022-457-F03C-32gp
GenBank Accession LN500224 [GenBank]
Sequencing primer gene specific primer, 32gp: 5'-CAGAATCCTTGAAAATCCAAATGT-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K112248-0022-457-F03C-32gp
GAGCTCTGCCTTATGCTTCAGCTGAACTACACAATCTGATAAAGAAACCTAGTACTAACA
TCATTATCTCACTTCGTTAATTCTCTAATCTAACTGTACGTTCCAAAATTTTAGTTTCTG
TATTACCGTAATTAAAAATTAGTTTCAAAATTTTACAGTCTAAACTTGTCAACCATCCCT
TGAAGCCATCTTAAGTGGTAAGTTCCTCTTCGCTTCTTTCTAATGGCTTTTCATCTTAAG
TGAAACGAACCTTTCCTAGTTCCATTCTTTGCGCTGATCCTTGTGGAGCGGATATCAGAG
ACGTCTCGAACTTGAAAAAGAAGCTGCCTCCGGAGTTGATGAAAGAAATGGGAACGATCA
GGTCATCTCATGACTGGGTAGCTACTTCAAAGGACGATGGAGTTCTGCGTCTCCAAGATG
ACTTAAACTCGGTTGCGTCGGACAGAGATCCAAGACGCAGCTTGCGACCTCATGCCTCAT
TGCCAAACCAAAATTGTTACCAACGTATTAATGCTGTTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 15-K112247-0022-457-F03C-8409
GenBank Accession LN493035 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K112247-0022-457-F03C-8409
GAGCCTTACATTGAACAGCATTAATACGTTGGTAACAATTTTGGTTTGGCAATGAGGCAT
GAGGTCGCAAGCTGCGTCTTGGATCTCTGTCCGACGCAACCGAGTTTAAGTCATCTTGGA
GACGCAGAACTCCATCGTCCTTTGAAGTAGCTACCCAGTCATGAGATGACCTGATCGTTC
CCATTTCTTTCATCAACTCCGGAGGCAGCTTCTTTTTCAAGTTCGAGACGTCTCTGATAT
CCGCTCCACAAGGATCAGCGCAAAGAATGGAACTAGGAAAGGTTCGTTTCACTTAAGATG
AAAAGCCATTAGAAAGAAGCGAAGAGGAACTTACCACTTAAGATGGCTTCAAGGGATGGT
TGACAAGTTTAGACTGTAAAATTTTGAAACTAATTTTTAATTACGGTAATACAGAAACTA
AAATTTTGGAACGTACAGTTAGATTAGAGAATTAACGAAGTGAGATAATGATGTTAGTAC
TAGGTTTCTTTATCAGATTGTGTAGTTCAGCTGAAGCATAAGGCAGAGCTCACATTGCGA
AATCAAAACCATATAATAAAAACATTTGG

Last Updated on 06.12.2018 16:11