Sequences from confirmation amplicon


Confirmation sequences for line 870C04 and hit At3g44600 are shown.
Line ID 870C04
Locus At3g44600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:16168776
Primers used for PCR KC83 - 8474, show primer details
   
Sequence Name 04-K103004-0022-870-C04C-OriL
GenBank Accession LN487872 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K103004-0022-870-C04C-OriL
GCCATTTACATTGACTCATTGCTGGTTTCTCCATATTTACATTTGAGGAAGTATGGATGG
TAATGTTTCTCATCTTTGTGATATTTATGATTTCATTCTGGTTTTGTAATGGCAAAGCAT
AACATATGTATATTTTGCATAATCTAGGTCACTTGAAATTTTGGAAGAAAAAGGGTGTTG
GTATCGAGTTTGCTAAGCATTTCCGCTCCCATCTCGGTCCAATTGAAGGTCTAGCGGTAA
GTATCGCGAGTGGTTTTTAAGATACTGTCACCTCAAATCCAGGCATATGCTCCATGTTTT
GAAAGATTAGCTTTTTATTTGTTTATTGAATGAAGATAGTTGACCTGGCGGGGAAATTAT
AGTTAGACATGTTATGGTTTCCGCAATTTTTTTGTTTGTGTCTAGTTCTATTATCAACCA
ATGACTCGTTTCAGCAATGCAGGTTAGCATTGATGGTTTGCTTTGCTGTACAATCTCAAA
TGATCATGCTGTTAAAATATATGATGTTGTCAACTACGATATGATGGCCATGATCCGTTT
GCCATATATTCCTGGTGCTGTTGAGTGGGTCTACAAGCAAGGGGATGTCAAAGCTAAACT
TGCTGTCAGTGACCGAGACTCTNTGTTTGTGC
   
Sequence Name 52-K103005-0022-870-C04C-KC83
GenBank Accession LN507780 [GenBank]
Sequencing primer gene specific primer, KC83: 5'-AGGATCATAAATGTGCACAAACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K103005-0022-870-C04C-KC83
AGTTAGCTTTGAATCCCCTTGGTTGTAGACCCACTCAACAGCACCAGGAATATATGGCAA
ACGGATCATGGCCATCATATCGTAGTTGACAACATCATATATTTTAACAGCATGATCATT
TGAGATTGTACAGCAAAGCAAACCATCAATGCTAACCTGCATTGCTGAAACGAGTCATTG
GTTGATAATAGAACTAGACACAAACAAAAAAATTGCGGAAACCATAACATGTCTAACTAT
AATTTCCCCGCCAGGTCAACTATCTTCATTCAATAAACAAATAAAAAGCTAATCTTTCAA
AACATGGAGCATATGCCTGGATTTGAGGTGACAGTATCTTAAAAACCACTCGCGATACTT
ACCGCTAGACCTTCAATTGGACCGAGATGGGAGCGGAAATGCTTAGCAAACTCGATACCA
ACACCCTTTTTCTTCCAAAATTTCAAGTGACCTAGATNATGCAAAATATACATATGTTAT
GCTTTGCCATTACAAAACCAGAATGAAATCATAAATATCACAAAGATGAGAAACATTACC
ATCCATACTTCCTCAAATGTAAATATGGAGAAACCAGCAATGAGTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGATGTCCGCAGCGTTATTATA
AAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAG
CAATAAACAAATTATTCTAATTCGGAAATCTNTATNTCGACGTGTCTA

Last Updated on 06.12.2018 16:11