Sequences from confirmation amplicon


Confirmation sequences for line 810E10 and hit At3g44670 are shown.
Line ID 810E10
Locus At3g44670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:16219267
Primers used for PCR 84we - 8474, show primer details
   
Sequence Name 83-K118585-0022-810-E10C-84we
GenBank Accession LN514357 [GenBank]
Sequencing primer gene specific primer, 84we: 5'-TTTTGAAGGCTCATGGGATA-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K118585-0022-810-E10C-84we
CATCAATGAGAGGCTTTTCAATCTTCTGTTGAATGCCTTTGGTCAGAAACAACCACATGA
AGGTTTCGATGAGATTGCATGGGAAGTTATGGCTTTAGCTGGTGAACTCCCTTTGGGATT
GAAGGTTCTAGGCTCTGCTTTGCGGGGGAAGTCCAAGCCAGAGTGGGAAAGAACACTACC
AAGGTTAAAAACTAGCCTCGACGGAAATATCGGGAGCATTATACAGTTCAGTTACGATGG
CTTATGTGACGAAGATAAATATCTCCTTCTTTATATAGCCTGCCTTTTTAACTACGAATC
AACAACCAAAGTGGAAGAGGTTCTTGCAAACAAGTTCTTGGATGTGAAACAAGGTCTTCA
CGTATTAGCTCAGAAATCTCTCATATCCATTGATGAGAATTCCTTATATGGAGACACAAT
AAATATGCATACTTTGCTCAGACAATTCGGAAGAGAAACTTCTCGTAAGCAATTCGTTTA
TCATGGCTTTACGAAACGTCAACTCTTGTTCAGGATATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 53-K118584-0022-810-E10C-8409
GenBank Accession LN508305 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K118584-0022-810-E10C-8409
TCGGAATNAGCCTTACATTGAATATATCCTGAACAAGAGTTGACGTTTCGTAAAGCCATG
ATAAACGAATTGCTTACGAGAAGTTTCTCTTCCGAATTGTCTGAGCAAAGTATGCATATT
TATTGTGTCTCCATATAAGGAATTCTCATCAATGGATATGAGAGATTTCTGAGCTAATAC
GTGAAGACCTTGTTTCACATCCAAGAACTTGTTTGCAAGAACCTCTTCCACTTTGGTTGT
TGATTCGTAGTTAAAAAGGCAGGCTATATAAAGAAGGAGATATTTATCTTCGTCACATAA
GCCATCGTAACTGAACTGTATAATGCTCCCGATATTTCCGTCGAGGCTAGTTTTTAACCT
TGGTAGTGTTCTTTCCCACTCTGGCTTGGACTTCCCCCGCAAAGCAGAGCCTAGAACCTT
CAATCCCAAAGGGAGTTCACCAGCTAAAGCCATAACTTCCCATGCAATCTCATCGAAACC
TTCATGTGGTTGTTTCTGACCAAAGGCATTCATACAGAAGATTTGAAAAGCCTCGTCATT
TGATGGATATTTCACTTTGTAAACATGATTTATCCCATGAGCCTTCAAAA

Last Updated on 06.12.2018 16:11