Sequences from confirmation amplicon


Confirmation sequences for line 500F01 and hit At3g48010 are shown.
Line ID 500F01
Locus At3g48010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17721410
Primers used for PCR GA02 - 8474, show primer details
   
Sequence Name 11-K025300-022-500-F01C-8409
GenBank Accession LN490947 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K025300-022-500-F01C-8409
CCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATACAAGAATTGATCCTGA
TCATCCAACTTTGAAGATGCCTAAAATGTTTAAACCAGAGGATCCAGGATTCTTCTGATA
GTTGTTGTGACAAATGCATTTAGGATTTCTGACACTAACTTGATTTGCTGTAACCAATAG
TCAGGTATACAAAGAAACATTATTACAGATTGAAGTAATTGTGACTTGACGTCGGTCGAT
ATTATGTCGATTTCTATCCAACGCACTCTTGGCTCCTACAGCATTTATTGGTACACAAAG
TGGGCTTTGAAACTCATAAAACACCTTGTGTTTAAGTCTAATTATGTTTTCACATAATTT
AGGTGAATTAAAAAATTAATTAATTATAAATTGAATTTGGGCAATGACCATATTTTGGTC
AAAAATAATGAGAATGACACTTATAAAAGAAAAATAAAGGATATAGTCATAGACTCATTC
CACTATTTGATTGGACGAAAATGATTTTTGATTACACACTTCAACTTGTATTTTTCTTCG
TCTA
   
Sequence Name 12-K025300-022-500-F01C-GA02
GenBank Accession LN491406 [GenBank]
Sequencing primer gene specific primer, GA02: 5'-GCACAAAATCATGTAGACGAAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K025300-022-500-F01C-GA02
ACAAGTTGAAGTGTGTAATCAAAAATCATTTTCGTCCAATCAAATAGTGGAATGAGTCTA
TGACTATATCCTTTATTTTTCTTTTATAAGTGTCATTCTCATTATTTTTGACCAAAATAT
GGTCATTGCCCAAATTCAATTTATAATTAATTAATTTTTTAATTCACCTAAATTATGTGA
AAACATAATTAGACTTAAACACAAGGTGTTTTATGAGTTTCAAAGCCCACTTTGTGTACC
AATAAATGCTGTAGGAGCCAAGAGTGCGTTGGATAGAAATCGACATAATATCGACCGACG
TCAAGTCACAATTACTTCAATCTGTAATAATGTTTCTTTGTATACCTGACTATTGGTTAC
AGCAAATCAAGTTAGTGTCAGAAATCCTAAATGCATTTGTCACAACAACTATCAGAAGAA
TCCTGGATCCTCTGGTTTAAACATTTTAGGCATCTTCAAAGTTGGATGATCAGGATCAAT
TCTTGTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTA
CGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAAT
Primers used for PCR 32vp - CR3S, show primer details
   
Sequence Name 28-K117953-0022-500-F01C-32vp
GenBank Accession LN499030 [GenBank]
Sequencing primer gene specific primer, 32vp: 5'-CACACCAGAATAACACCACATTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K117953-0022-500-F01C-32vp
TAGAACCTAATTTTCGTTCTTTTATTTTTAGGTGCCGTTCTTTGCGCAGATGGATGACCA
GCTCTTGGATGCGATATGTGAGCGTCTAGTTCCGTCCTTGAACACAAAAGACACCTATGT
TATACGCGAAGGCGATCCTGTAAACGAGATGCTTTTCATTATTAGAGGTCAGATGGAGAG
TTCAACTACAGATGGTGGACGGTCAGGATTCTTTAACTCTATCACACTTAGACCAGGAGA
TTTCTGCGGTGAAGAGCTTTTAACCTGGGCTTTAGTGCCAAACATTAATCATAATCTACC
ATTGTCCACGAGAACCGTAAGGACTCTCTCCGAAGTAGAAGCTTTCGCTCTCCGAGCAGA
GGACTTGAAATTCGTAGCTAACCAATTCAGACGTCTTCACAGCAAGAAACTCCAACATGC
TTTCAGGTAATCCTCGTATGGTCTCATAAGCAATATAGGTTTCTTGCTTCACACGCTATA
AGGTTTCTTGATCTTTATGTTTTGACTGTAACCAGATACTATTCGCATCAATGGAGAGCA
TGGGGAACTTGTTTTATACAAGCTGCTTGGAGGCGATACATGAAGAGGAAATTAGCAATG
GAGTTAGCTAGGCAAGAGGAAGAAGACGATTACTTCTATGACGATGATGGAGACTATCAA
TTCGAAGAGGACATGCCTGAAAGTAATAATAACAACGGAGACGAAAACAGCAGCAATAAT
CAAAATTTGAGTGCAACAATATTAGCGTCTAAGTTCGCAGCTAATACTAAGAGAGGTGTT
TTAGGAATTAGAAATTTTATTGATAGAAGTATTTTACAAATACAAATACATACTAAGGGT
TTC
   
Sequence Name 04-K117952-0022-500-F01C-CR3S
GenBank Accession LN488100 [GenBank]
Sequencing primer T-DNA primer, CR3S: 5'-TTGAGCATATAAGAAACCCTTAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K117952-0022-500-F01C-CR3S
CATAAATTTCAATTCCTAAAACACCTCTCTTAGTATTAGCTGCGAACTTAGACGCTAATA
TTGTTGCACTCAAATTTTGATTATTGCTGCTGTTTTCGTCTCCGTTGTTATTATTACTTT
CAGGCATGTCCTCTTCGAATTGATAGTCTCCATCATCGTCATAGAAGTAATCGTCTTCTT
CCTCTTGCCTAGCTAACTCCATTGCTAATTTCCTCTTCATGTATCGCCTCCAAGCAGCTT
GTATAAAACAAGTTCCCCATGCTCTCCATTGATGCGAATAGTATCTGGTTACAGTCAAAA
CATAAAGATCAAGAAACCTTATAGCGTGTGAAGCAAGAAACCTATATTGCTTATGAGACC
ATACGAGGATTACCTGAAAGCATGTTGGAGTTTCTTGCTGTGAAGACGTCTGAATTGGTT
AGCTACGAATTTCAAGTCCTCTGCTCGGAGAGCGAAAGCTTCTACTTCGGAGAGAGTCCT
TACGGTTCTCGTGGACAATGGTAGATTATGATTAATGTTTGGCACTAAAGCCCAGGTTAA
AAGCTCTTCACCGCAGAAATCTCCTGGTCTAAGTGTGATAGAGTTAAAGAATCCTGACCG
TCCACCATCTGTAGTTGAACTCTCCATCTGACCTCTAATAATGAAAAGCATCTCGTTTAC
AGGATCGCCTTCGCGTATAACATAGGTGTCTTTTGTGTTCAAGGACGGAACTAGACGCTC
ACATATCGCATCCAAGAGCTGGTCATCCATCTGCGCAAAGAACGGCACCTGAAAAATTAA
AAGAACGTAAAATTAGGGTTTCTTACTTGAGTGGAACGTAATGGAAATGTGGTGTATT

Last Updated on 06.12.2018 16:11