Sequences from confirmation amplicon


Confirmation sequences for line 336E01 and hit At3g48420 are shown.
Line ID 336E01
Locus At3g48420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17931558
Primers used for PCR BV43 - 8474, show primer details
   
Sequence Name 91-K105954-0022-336-E01C-BV43
GenBank Accession LN515122 [GenBank]
Sequencing primer gene specific primer, BV43: 5'-ATGGATTTGTGTATGTGCAGATGT-3'
Sequence (A. th genome BLAST matches underlined) 
>91-K105954-0022-336-E01C-BV43
CGATGGGCTAAAGCTGCAAAGCTGCGGGAATGACTTGTATAGTTACAAAGAGTGGGTGAG
TTTTAGTCTGAAATCAGAATCCTATTCATAATTCCATAACAATCAGAATTGAAATGATCA
TTCGTTCTTTCATCTTTGGAATTGTAGATACACGGCTGATGAAGATTTCGAGAACGCAGA
TGCGGTTTTCGACTGCATTGGAGACCCTCCAGAAGAGAGATTTGATTTGGCATTCTGTGG
AAGTCTTCTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCG
   
Sequence Name 27-K105955-0022-336-E01C-8409
GenBank Accession LN498391 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K105955-0022-336-E01C-8409
ATTTACATTGAATATATCCTGAGAAGACTTCCACAGAATGCCAAATCAAATCTCTCTTCT
GGAGGGTCTCCAATGCAGTCGAAAACCGCATCTGCGTTCTCGAAATCTTCATCAGCCGTG
TATCTACAATTCCAAAGATGAAAGAACGAATGATCATTTCAATTCTGATTGTTATGGAAT
TATGAATAGGATTCTGATTTCAGACTAAAACTCACCCACTCTTTGTAACTATACAAGTCA
TTCCCGCAGCTTTTGCAGCTGCTAGCCCGATCGCGCTGTCTTCAACCACTACACATCTGC
ACATACACAAATCCA
Primers used for PCR 31au - 3144, show primer details
   
Sequence Name 03-K106829-0022-336-E01C-3144
GenBank Accession LN487504 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K106829-0022-336-E01C-3144
CCTTGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCG
GGGATCAGATTGTCGTTTCCCGCCTTCGGTTCAAACCACATTAAGAAAATTGTTTCTATA
TCAACATTGTTGTGTATAGTCATTTGAACAGAACATTCATGTAAAACCATATATATAATA
TAATTCGATTATAACATTATGAAGAAAAGCGTTACAATATATTGGGCTTGTGAGCGTTGT
TGGGTTTACTATGTAAATGGTTTTGCTTGACCATTGTTGCAAATTTCTATGTGAACTTCG
AATATACAACAAACGCCGAAACACAAAGCTTCGAATATACAACAAACGACGGGACAACGT
AAAGTTCGTACCTTTTTCAAGTTGGTATCTGAATTTTGGTGAATTGAGTTTTATCTCCAT
AAAGCTACGAAATTGC
   
Sequence Name 08-K106830-0022-336-E01C-31au
GenBank Accession LN489750 [GenBank]
Sequencing primer gene specific primer, 31au: 5'-AGCAATTTCGTAGCTTTATGGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K106830-0022-336-E01C-31au
CTTGAAAAGGACGAACTTTACGTTGTCCCGTCGTTTGTTGTATATTCGAAGCTTTGTGTT
TCGGCGTTTGTTGTATATTCGAAGTTCACATAGAAATTTGCAACAATGGTCAAGCAAAAC
CATTTACATAGTAAACCCAACAACGCTCACAAGCCCAATATATTGTAACGCTTTTCTTCA
TAATGTTATAATCGAATTATATTATATATATGGTTTTACATGAATGTTCTGTTCAAATGA
CTATACACAACAATGTTGATATAGAAACAATTTTCTTAATGTGGTTTGAACCGAAGGCGG
GAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATA
TAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGA
TATCACATCAATCCA

Last Updated on 06.12.2018 16:11