Sequences from confirmation amplicon


Confirmation sequences for line 180H10 and hit At3g48860 are shown.
Line ID 180H10
Locus At3g48860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18120330
Primers used for PCR 09fx - 8474, show primer details
   
Sequence Name 23-K111683-0022-180-H10C-09fx
GenBank Accession LN496613 [GenBank]
Sequencing primer gene specific primer, 09fx: 5'-TGTGGTGTTATAGCTCGCAAATAC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K111683-0022-180-H10C-09fx
CAACTCAACTTTCTCTGCTGGGCGAATATAGCACAACAGCATCCTCCACCTGATATTCAA
AAGAGAGAAGAAGATTTGAAAACGTAGTCCTTGGAGATATCTGCTAGGTGACAACACTTG
TAGGTAACTCAGAAAGAATTATGGATACCTTTAATGATGCTAGCTCTCTCAAACCCATCT
CAACTGAAAGCATACTTTCAATATTTCCTTCTCCAGTTAGATCACTCAAATCTCACCTGC
ATGTCCGGGCATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 07-K111682-0022-180-H10C-8409
GenBank Accession LN489334 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K111682-0022-180-H10C-8409
CATTTCAATTGAATATGCCCGGACATGCAGGTGAGATTTGAGTGATCTAACTGGAGAAGG
AAATATTGAAAGTATGCTTTCAGTTGAGATGGGTTTGAGAGAGCTAGCATCATTAAAGGT
ATCCATAATTCTTTCTGAGTTACCTACAAGTGTTGTCACCTAGCAGATATCTCCAAGGAC
TACGTTTTCAAATCTTCTTCTCTCTTTTGAATATCAGGTGGAGGATGCTGTTGTGCTTAT
ATTCGCCCAGCAGAGAAAGTTGAGTTTGGTTCGCCACACTGTTTCCGGTATGTAATGTAT
TTGCGAGCTATAACACCAC

Last Updated on 06.12.2018 16:11