Sequences from confirmation amplicon


Confirmation sequences for line 543A03 and hit At3g49970 are shown.
Line ID 543A03
Locus At3g49970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18529028
Primers used for PCR 26wj - 8474, show primer details
   
Sequence Name 67-K118585-0022-543-A03C-26wj
GenBank Accession LN511908 [GenBank]
Sequencing primer gene specific primer, 26wj: 5'-CAAGAATCTCACCAACCCTCTAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K118585-0022-543-A03C-26wj
GACCAAGTGAGTATCTCTTAAACCCTCTAGCGATCATCGCGACCAAGACACGTCGAAACA
TATCAATCTTTAGAACAGCTAAATCGTCAGCCCACCAGTCAACTATATCCTCATTGCTCT
GGCTCTCCTGGCATATTGCATAAGCGATTGAATCGATACACCGGTCCACGAGTCTCACTC
TCTCGGCTATAGGCAACAAATCTTGAGATTTTTGCAGAACTTTAACTGATTTTGATAAAC
TCTTGAGGATCACTTCGTTTAAGTAAACCTCTATTGTCTCCACTAGGTTTTCAACAGAGT
GTTCCTCTGTCATCTCTAGATACTCTGCTGCACATAGTAGCATCGCAATGTTCTCTGTGT
TTATCTCGAAGCTTATGTCGTAGCAGAATTTGATGACTAGCTCAAATCCTTCTGCACCTC
CAGGAAAATCTGGGATTCTGATAATGTTGGAATCATTGCTTGATTCAGAAGCAAGTTTCT
TTATAAACCCACATTTTGAAACAAGTGGAAACTGCAGATTATCACAGAGACTATGTATAT
GAGAAAGTGATCTCCTTGTGTATAATTTAGTAGATCTTGAAAAAATAAAATCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 37-K118584-0022-543-A03C-8409
GenBank Accession LN502790 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K118584-0022-543-A03C-8409
TCATTGATTTATTTTTTCAGATCTACTAAATTATACACAAGGAGATCACTTTCTCATATA
CATAGTCTCTGTGATAATCTGCAGTTTCCACTTGTTTCAAAATGTGGGTTTATAAAGAAA
CTTGCTTCTGAATCAAGCAATGATTCCAACATTATCAGAATCCCAGATTTTCCTGGAGGT
GCAGAAGGATTTGAGCTAGTCATCAAATTCTGCTACGACATAAGCTTCGAGATAAACACA
GAGAACATTGCGATGCTACTATGTGCAGCAGAGTATCTAGAGATGACAGAGGAACACTCT
GTTGAAAACCTAGTGGAGACAATAGAGGTTTACTTAAACGAAGTGATCCTCAAGAGTTTA
TCAAAATCAGTTAAAGTTCTGCAAAAATCTCAAGATTTGTTGCCTATAGCCGAGAGAGTG
AGACTCGTGGACCGGTGTATCGATTCAATCGCTTATGCAATATGCCAGGAGAGCCAGAGC
AATGAGGATATAGTTGACTGGTGGGCTGACGATTTAGCTGTTCTAAAGATTGATATGTTT
CGACGTGTCTTGGTCGCGATGATCGCTAGAGGGTTTAAGAGATACTCACTTGGTCCAGTA
CTTAAACTCTATGCCGAAAAAGCTCTTAGAGGGTTGGGAAGAATTCTTG

Last Updated on 06.12.2018 16:11