Sequences from confirmation amplicon
Confirmation sequences for line 347C07 and hit At3g50840 are shown.
Primers used for PCR |
17vc - 8474, show primer details |
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Sequence Name |
54-K117671-0022-347-C07C-17vc |
GenBank Accession |
LN508610 [GenBank] |
Sequencing primer
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gene specific primer, 17vc: 5'-ACAAGCATTCTTTCTCTAGCTGGT-3' |
Sequence (A. th genome BLAST matches underlined)
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>54-K117671-0022-347-C07C-17vc
CAACGAAGCACCTGATTCTCCTTCACCGCTTCCTTCCATCTACCCAAGTCCACCACCNGT
TTTATCACCGNTCTTCCTCCGTCAGTAAAGTCCCAGTGATTACTTGCCGGAGCTGTAGCT
GCTCCAAAAACAGAACTTGTACGACGGCTCGTAGTGGTAAACGCTCGTTTTGTGCCGCGT
GTGTGCATCCTTCAACAGTCAGTTTTCTACAATCCATCACGCTACAAATCTTCTCCTTCT
CTTCCTCCAATATCCATGAATGAGTCTATAAAAACAAAAATCACAACTCAATCATCAGGT
GCTGTGGTGTAGTGGTTATCACGTTTGCCTTACACGCAAAAGGTCTCCAGTTCGACCCTG
GGCAGCACCAAACCATAGACAATTTTATAAATTTTTATAATTAATTACCTTGAAATATAT
ATCGACGGCTCTGTATAGTCCATCATCGTAAACCCGTGCTTGAACCGGAAGCAAAACCGC
CAGATTATAAAACTGTTCCGGTTTAAGATTAGCGTCCGATGCAATTTCACCGAGAACTCC
GTCGATCAATCTCCCAACCACCGTCAATGACGACGACGACACGGCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
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Primers used for PCR |
40vk - 8474, show primer details |
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Sequence Name |
72-K118684-0022-347-C07C-40vk |
GenBank Accession |
LN512808 [GenBank] |
Sequencing primer
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gene specific primer, 40vk: 5'-CCTCCAATATCCATGAATGAGTCT-3' |
Sequence (A. th genome BLAST matches underlined)
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>72-K118684-0022-347-C07C-40vk
ATCACTCATCATCAGGTGCTGTGGTGTAGTGGTTATCACGTTTGCCTTACACGCAAAAGG
TCTCCAGTTCGACCCTGGGCAGCACCAAACCATAGACAATTTTATAAATTTTTATAATTA
ATTACCTTGAAATATATATCGACGGCTCTGTATAGTCCATCATCGTAAACCCGTGCTTGA
ACCGGAAGCAAAACCGCCAGATTATAAAACTGTTCCGGTTTAAGATTAGCGTCCGATGCA
ATTTCACCGAGAACTCCGTCGATCAATCTCCCAACCACCGTCAATGACGACGACGACACG
GCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCAGCACGTTATT
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Primers used for PCR |
CT18 - 3144, show primer details |
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Sequence Name |
25-K119622-0022-347-C07C-CT18 |
GenBank Accession |
LN497688 [GenBank] |
Sequencing primer
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gene specific primer, CT18: 5'-TGTGATCTTATCCATGAAGTCGAG-3' |
Sequence (A. th genome BLAST matches underlined)
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>25-K119622-0022-347-C07C-CT18
TCTACTTCTTCGCGAAGATACCGTTCCAGGAATCTCGCGTGCATCGTCAGCATCTTCATC
TTCTTCATCTTCTTCCACCACCATAGCTTCCGAGAATCAACAAAGAGAATTATTAGAGAC
GATAACTTCAGATCTTCCATTAACTGCGACGACTACGAGATCTCTCTTCGGTTTGTTACG
AGCCGCGATCATTCTCAACGCCTCCGAGAATTGCAGGAAGTTTCTAGAGAAAAAAATCGG
ATCGAATCTTGAGAAAGCTACGCTTGATGATCTTCTCATTCCAAGTTACTCGTATCTCAA
CGAAACGTTATACGATATTGATTTGGTGGAGAGACAATCTGATCCCCGGGTACCCTGTCC
TCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATT
GTGCGTCATCCCTTACGTCAGTGGAGATATCAATCCAATCCA
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Sequence Name |
01-K119620-0022-347-C07C-3144 |
GenBank Accession |
LN486808 [GenBank] |
Sequencing primer
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T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3' |
Sequence (A. th genome BLAST matches underlined)
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>01-K119620-0022-347-C07C-3144
CGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGG
GATCAGATTGTCTCTCCACCAAATCAATATCGTATAACGTTTCGTTGAGATACGAGTAAC
TTGGAATGAGAAGATCATCAAGCGTAGCTTTCTCAAGATTCGATCCGATTTTTTTCTCTA
GAAACTTCCTGCAATTCTCGGAGGCGTTGAGAATGATCGCGGCTCGTAACAAACCGAAGA
GAGATCTCGTAGTCGTCGCAGTTAATGGAAGATCTGAAGTTATCGTCTCTAATAATTCTC
TTTGTTGATTCTCGGAAGCTATGGTGGTGGAAGAAGAAGAAGAAGATGAAGATGCTGACG
ATGAACGCGAGATTCCTGGAATGTGTTTCTTCGCGTAAGTAAGTAGAGATCTCTCTACAA
TCTCAGGACTCAGGACACTCGACTTCATGGAAAAGATCACAAA
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Primers used for PCR |
91wr - 3144, show primer details |
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Sequence Name |
26-K119622-0022-347-C07C-91wr |
GenBank Accession |
LN498153 [GenBank] |
Sequencing primer
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gene specific primer, 91wr: 5'-CCGAGTATCTAGAAATGACGGAAG-3' |
Sequence (A. th genome BLAST matches underlined)
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>26-K119622-0022-347-C07C-91wr
AACGAGAATTTCTTTCAGAGTTCGTCTTCCTAACGTACAAGAATCCATCAAAGCGTTGAA
AGCTTGTGAATCAGTCTCGTCTTTAGCTGAATCACTCTGTATAACGGAACAGTGTATAGA
CTCCATCGTCTTCCAAGCTTCCTCAACCGATCCGTCGTCGTTTTACGGTTGGCCAATAAA
CAACGGAGGTATATTCACCGTTGACCGGAAGAAACAGAGCAAAGATTCGAAAACAGAGCT
TTGGTTCGAAGATCTAACGGAGTTGAGTTTTCCAATCTTCAGACGTGTGATCTTATCCAT
GAAGTCGAGTGTCCTGAGTCCTGAGATTGTAGAGAGATCTCTACTTACTTACGCGAAGAA
ACACATTCCAGGAATCTCGCGTTCATCGTCAGCATCTTCATCTTCTTCTTCTTCTTCCAC
CACCATAGCTTCCGAGAATCAACAAAGAGAATTATTAGAGACGATAACTTCAGATCTTCC
ATTAACTGCGACGACTACGAGATCTCTCTTCGGTTTGTTACGAGCCGCGATCATTCTCAA
CGCCTCCGAGAATTGCAGGAAGTTTCTAGAGAAAAAAATCGGATCGAATCTTGAGAAAGC
TACGCTTGATGATCTTCTCATTCCAAGTTACTCGTATCTCAACGAAACGTTATACGATAT
TGATTTGGTGGAGAGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAAC
TTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGT
CAGTGGAG
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Sequence Name |
02-K119620-0022-347-C07C-35St |
GenBank Accession |
LN487262 [GenBank] |
Sequencing primer
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T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3' |
Sequence (A. th genome BLAST matches underlined)
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>02-K119620-0022-347-C07C-35St
TTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGG
GGATCAGATTGTCTCTCCACCAAATCAATATCGTATAACGTTTCGTTGAGATACGAGTAA
CTTGGAATGAGAAGATCATCAAGCGTAGCTTTCTCAAGATTCGATCCGATTTTTTTCTCT
AGAAACTTCCTGCAATTCTCGGAGGCGTTGAGAATGATCGCGGCTCGTAACAAACCGAAG
AGAGATCTCGTAGTCGTCGCAGTTAATGGAAGATCTGAAGTTATCGTCTCTAATAATTCT
CTTTGTTGATTCTCGGAAGCTATGGTGGTGGAAGAAGAAGAAGAAGATGAAGATGCTGAC
GATGAACGCGAGATTCCTGGAATGTGTTTCTTCGCGTAAGTAAGTAGAGATCTCTCTACA
ATCTCAGGACTCAGGACACTCGACTTCATGGATAAGATCACACGTCTGAAGATTGGAAAA
CTCAACTCCGTTAGATCTTCGAACCAAAGCTCTGTTTTCGAATCTTTGCTCTGTTTCTTC
CGGTCAACGGTGAATATACCTCCGTTGTTTATTGGCCAACCGTAAAACGACGACGGATCG
GTTGAGGAAGCTTGGAAGACGATGGAGTCTATACACTGTTCCGTTATACAGAGTGATTCA
GCTAAAGACGAGACTGATTCACAAGCTTTCAACGCTTTGATGGATTCTTGTACGTTAGTG
AAGACGAACTCTGAAAGAAACTTCTCTGTTTTTGAAATGAGATTCTCCGGTGAGTACTCT
TCCGTCAT
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Last Updated on 06.12.2018 16:11 |