Sequences from confirmation amplicon


Confirmation sequences for line 053A09 and hit At3g52526 are shown.
Line ID 053A09
Locus At3g52526 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19485120
Primers used for PCR 05pk - 8474, show primer details
   
Sequence Name 27-K115038-0022-053-A09C-05pk
GenBank Accession LN498519 [GenBank]
Sequencing primer gene specific primer, 05pk: 5'-CAGGGATGTAAGACCTCAAAACAT-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K115038-0022-053-A09C-05pk
TCTTTCTGTTTGAGAATATCGATCCCTGAGGGAGAATCATCTGTGTATGATGATAAAGTA
GTTTATGGGGTATCCGTTGATCCGGAGTATAATGGAACAGGTTTAGTTTCCGAAAAGTTT
GATGTCTATAGCTTTGGAGTTACAATGCTGTTTCTTCTAGGCGGAGAGCTTGGCCTAACC
TGGTTATCTGCTATCATTGGGGAGTTTGGATTCCCATTTCCTGGTTGCAGGATATATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 17-K115037-0022-053-A09C-8409
GenBank Accession LN493973 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K115037-0022-053-A09C-8409
TTGAATATATCCTGCAACCAGGAAATGGGAATCCAAACTCCCCAATGATAGCAGATAACC
AGGTTAGGCCAAGCTCTCCGCCTAGAAGAAACAGCATTGTAACTCCAAAGCTATAGACAT
CAAACTTTTCGGAAACTAAACCTGTTCCATTATACTCCGGATCAACGGATACCCCATAAA
CTACTTTATCATCATACACAGATGATTCTCCCTCAGGGATCGATATTCTCAAACAGAAAG
AAGAGAGCTTCGCAGTCCCATCTTCGTCCAAGAATATGTTTTGAGGTCTTACATCCCTGA
G

Last Updated on 06.12.2018 16:11