Sequences from confirmation amplicon


Confirmation sequences for line 110A04 and hit At3g52700 are shown.
Line ID 110A04
Locus At3g52700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19538535
Primers used for PCR 46fb - 8474, show primer details
   
Sequence Name 45-K115714-0022-110-A04C-46fb
GenBank Accession LN505640 [GenBank]
Sequencing primer gene specific primer, 46fb: 5'-GGTGATCGTTTTGGAGAATAAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K115714-0022-110-A04C-46fb
TAAGATTTTGAGACCATAGTGTACGTAACGATATTGAAGTTTCAAACTTAATACATTATT
AATTTTTCTGGACGATATTCAAACCAATTTGAGTGTCTTTTGGGCTCCACAACCCCTGCT
TTTAAGCTAAACCCTTTATGTCAAACTTGTTATTCACGTTTGAATGAATAGAGAAGTCAA
TATATACGATTGATAACATCTAGCTAGCTACTACTTTCATGTTTATTTGACATTTTTGTA
TGGAAACGGTCCAAATTAACGTGACATCGTGATTTATGATTGCCGTGTATAATAAATTAA
ATGCAGGTGAGGTCAATGTCAAAGGAACAAGTAGAACTACTCAGATCTCCATTGGACACT
TCTTTCAAGAACAATTCAAGACCGTGTCAACCCCTCAACGGCCGGAGAGTTGAGATGTTC
CACGACCACGAGCGTGTCGATNAAAAAGAAACCGGTAACAAAAAGAAAAAACCCAATTAA
AATAGTTTTACATTGTCTATTGGTTTGTTTAGAACCCTAATTAGCTTTGTAAAACTAATA
ATCTCTTATGTAGTACTGTGTTGTTGTTTACGACTTGATATACGATTTCCAAATTTACTA
TGTGGTGTTGTGAGATATGTTCTACATTGAGACAACGTGAATAACTAATATCATATTTAG
TATAATAAGAAGTTAAGAACAAATGAATTAGATGTCGAANAAATTAATTTGGTTCCACGT
TTTAATGTCATCGAATACAACGGTTACATTAATATCCTCTACATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCATTCANAATGT
   
Sequence Name 05-K115713-0022-110-A04C-8409
GenBank Accession LN488486 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K115713-0022-110-A04C-8409
ATGTAACCGTTGTATTCGATGACATTAAAACGTGGAACCAAATTAATTTTTTCGACATCT
AATTCATTTGTTCTTAACTTCTTATTATACTAAATATGATATTAGTTATTCACGTTGTCT
CAATGTAGAACATATCTCACAACACCACATAGTAAATTTGGAAATCGTATATCAAGTCGT
AAACAACAACACAGTACTACATAAGAGATTATTAGTTTTACAAAGCTAATTAGGGTTCTA
AACAAACCAATAGACAATGTAAAACTATTTTAATTGGGTTTTTTCTTTTTGTTACCGGTT
TCTTTTTTATCGACACGCTCGTGGTCGTGGAACATCTCAACTCTCCGGCCGTTGAGGGGT
TGACACGGTCTTGAATTGTTCTTGAAAGAAGTGTCCAATGGAGATCTGAGTAGTTCTACT
TGTTCCTTTGACATTGACCTCACCTGCATTTAATTTATTATACACGGCAATCATAAATCA
CGATGTCACGTTAATTTGGACCGTTTCCATACAAAAATGTCAAATAAACATGAAAGTAGT
AGCTAGCTAGATGTTATCAATCGTATATATTGACTTCTCTATTCATTCAAACGTGAATAA
CAAGTTTGACATAAAGGGTTTAGCTTAAAAGCAGGGGTTGTGGAGCCCAAAAGACACTCA
AATTGGTTTGAATATCGTCCAGAANAATTAATAATGTATTAAGTTTGAAACTTCAATATC
GTTACGTACACTATGGTCTCAAAATCTTTAATGTGCTAGAATCAATTTTCGGTGGAATTT
AAATGTTTTTATTCTCCAAA

Last Updated on 06.12.2018 16:11