Sequences from confirmation amplicon


Confirmation sequences for line 142B06 and hit At3g52720 are shown.
Line ID 142B06
Locus At3g52720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19539024
Primers used for PCR 46fb - 8474, show primer details
   
Sequence Name 43-K110825-0022-142-B06C-46fb
GenBank Accession LN504872 [GenBank]
Sequencing primer gene specific primer, 46fb: 5'-GGTGATCGTTTTGGAGAATAAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K110825-0022-142-B06C-46fb
AGATTTTGAGACCATAGTGTACGTAACGATATTGAAGTTTCAAACTTAATACATTATTAA
TTTTTCTGGACGATATTCAAACCAATTTGAGTGTCTTTTGGGCTCCACAACCCCTGCTTT
TAAGCTAAACCCTTTATGTCAAACTTGTTATTCACGTTTGAATGAATAGAGAAGTCAATA
TATACGATTGATAACATCTAGCTAGCTACTACTTTCATGTTTATTTGACATTTTTGTATG
GAAACGGTCCAAATTAACATGTAAATTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCAAAGTTATTA
   
Sequence Name 27-K110824-0022-142-B06C-8409
GenBank Accession LN498441 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K110824-0022-142-B06C-8409
GCCATTAATTTCATGTTAATTTGGACCGTTTCCAACAAAAATGTCAAATAAACATGAAAG
TAGTAGCTAGCTAGATGTTATCAATCGTATATATTGACTTCTCTATTCATTCAAACGTGA
ATAACAAGTTTGACATAAAGGGTTTAGCTTAAAAGCAGGGGTTGTGGAGCCCAAAAGACA
CTCAAATTGGTTTGAATATCGTCCAGAAAAATTAATAATGTATTAAGTTTGAAACTTCAA
TATCGTTACGTACACTATGGTCTCAAAATCTTTAATGTGCTAGAATCAATTTTCGGTGGA
ATTTAAATGTTTTTATTCTCCAAAACGATCAC

Last Updated on 06.12.2018 16:11