Sequences from confirmation amplicon


Confirmation sequences for line 816E03 and hit At3g54010 are shown.
Line ID 816E03
Locus At3g54010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20005188
Primers used for PCR PH15 - 8474, show primer details
   
Sequence Name 45-K028245-022-816-E03C-PH15
GenBank Accession LN505410 [GenBank]
Sequencing primer gene specific primer, PH15: 5'-AACTATCTCAACCAAATGTCCACC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K028245-022-816-E03C-ph5
ATCGAGTTCATTAAGGCTACTTATGGTTTGGGCTTGTTTTGCCAATTTTGTTACTTAATA
TAAGATCTCTGAGTTGTTTTCTTTTGAGCTCACAGGAAGCGGAGAGCAAGGCTAGAAAAC
AGTTCAAAGGATTATTCGACAAGAGACCGGGGGAGATAACCGAAGTTGGTTCAGAAATAA
GAGAGGAGTCTAAAACTATAGAAGAAGTAGATGAGACAAAGGATAATGATGATGATGAAA
CACTGGAAGAAGAAGGAGCTACGACAGTGAGCACGGAAAGGAAGAGGAAATGGTCGGAGA
AAGCCTGGCCGTTTCTGAAGAACGTGATGCTCCAAATCGGAATCCAGCTTGGTGTTGTTC
TGATAGGGATTTTGATTTTCCAGTTTGTGAGCGCCAAATTTACATGAGAGAGATATGAAT
GAATAAACAAACCAACGGCACTGTAGACTCAGAAAATATAAAACTAATGGATTAAACGTT
AAGCTTAATGTATTTTTGTTTGGTTTAGTTTATGAAAGTAGTTGCTCAAAGATGGAATTA
TTTGCACCACTACACGATCCAAATGGCTTCATGTCCGGAAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAAT
TACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAA
   
Sequence Name 44-K028245-022-816-E03C-8409
GenBank Accession LN505062 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K028245-022-816-E03C-8409
CATGTAGATTTTCCGGACATGAAGCCATTTGGATCGTGTAGTGGTGCAAATAATTCCATC
TTTGAGCAACTACTTTCATAAACTAAACCAAACAAAAATACATTAAGCTTAACGTTTAAT
CCATTAGTTTTATATTTTCTGAGTCTACAGTGCCGTTGGTTTGTTTATTCATTCATATCT
CTCTCATGTAAATTTGGCGCTCACAAACTGGAAAATCAAAATCCCTATCAGAACAACACC
AAGCTGGATTCCGATTTGGAGCATCACGTTCTTCAGAAACGGCCAGGCTTTCTCCGACCA
TTTCCTCTTCCTTTCCGTGCTCACTGTCGTAGCTCCTTCTTCTTCCAGTGTTTCATCATC
ATCATTATCCTTTGTCTCATCTACTTCTTCTATAGTTTTAGACTCCTCTCTTATTTCTGA
ACCAACTTCGGTTATCTCCCCCGGTCTCTTGTCGAATAATCCTTTGAACTGTTTTCTAGC
CTTGCTCTCCGCTTCCTGTGAGCTCAAAAGAAAACAACTCAGAGATCTTATATTAAGTAA
CAAAATTGGCAAAACAAGCCCAAACCATAAGTAGCCTTAATGAACTCGATTCGGGTGGAC
ATTT

Last Updated on 06.12.2018 16:11