Sequences from confirmation amplicon


Confirmation sequences for line 016D04 and hit At3g55000 are shown.
Line ID 016D04
Locus At3g55000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20383134
Primers used for PCR DC48 - 3144, show primer details
   
Sequence Name 41-K032604-022-016-D04C-3144
GenBank Accession LN503974 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K032604-022-016-D04C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTTACTATCTAAATGGCGTACCCAGA
TGTCTTTTTTCTTCAAATAAATGGGCAGGCCGGTTTCTGCATCTCAAGCATCGGGTGAGT
CATCATTTCCCTAGTTTCTTGAAGTATTGGTTGAAATACGTATGCTTCATTATTTTTTGA
TACCAAATAAATATGACAAGATGTTACAGATAGAAGAGCCGGGTTGTCTACATCTGGGTA
CAGAAAAGATGAATTCAACTGGAGACAAGGTAACCAAGATACACATGAAGAGGTAACAAG
AGCTTCAGCGGCGCTGGAAAATCTACAGCTTGATAGGAAAACTCGGAACTTAACATCGTC
TTGGAGGTAAGCTTTCAAGGAAGTCTCATTGAATCCCACATTGAAATGA
   
Sequence Name 41-K032604-022-016-D04C-DC48
GenBank Accession LN503975 [GenBank]
Sequencing primer gene specific primer, DC48: 5'-CAACTTGCTTTGTTTTCATTTCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K032604-022-016-D04C-DC48
TCTTGAGCTTACCTCCAGACGATGTTAAGTTCCGAGTTTTCCTATCAAGCTGTAGATTTT
CCAGCGCCGCTGAAGCTCTTGTTACCTCTTCATGTGTATCTTGGTTACCTTGTCTCCAGT
TGAATTCATCTTTTCTGTACCCAGATGTAGACAACCCGGCTCTTCTATCTGTAACATCTT
GTCATATTTATTTGGTATCAAAAAATAATGAAGCATACGTATTTCAACCAATACTTCAAG
AAACTAGGGAAATGATGACTCACCCGATGCTTGAGATGCAGAAACCGGCCTGCCCATTTA
TTTGAAGAAAAAAGACATCTGGGTACGCCATTTAGATAGTAAAGGCGGGAAACGACAATC
TGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGT
CTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAAT
CCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGT
Primers used for PCR FP58 - 3144, show primer details
   
Sequence Name 60-K035347-022-016-D04C-FP58
GenBank Accession LN510185 [GenBank]
Sequencing primer gene specific primer, FP58: 5'-CTCGCAACCAAGAGAAGAAGTTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K035347-022-016-D04C-FP58
AGAAAAAAAGTCCCCTTAGTAGAGATTAGAGTTCTTCAGAGATTTGAAAAACACGTTTAG
CAAATTTCCTAGCCTCAAGTTCTCTTCACATCTAAGAATCATGTTTCCATCCTATCATAG
TTAAAACCATTATCATCTGTTTTTGCCGTATGCTCCTCAGATGTAGTGATCAACTTTTTT
TTGTAATGAATTTTTTCTTCAGAGCATGACGCAGGGCATGGGTAGTAGTTCAAGGAGAGA
TTCAGAAACAGAGTCGTCATCAAGCCTTGAGTCCAGAAATCCTCCTCGCAGATCATCTGC
TTCTGATAGCTTACCTCCTCAACGAAGGTACACATATCTTCAACTTCTTATTATTATGCC
AAATCAAAGATCGTTACTCTTCGACATGATTGCCAATTTTAACCGAAGGCGGGAAACGAC
AATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAA
GGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACAT
CAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTG
GGTGGG
   
Sequence Name 12-K035347-022-016-D04C-3144
GenBank Accession LN491480 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K035347-022-016-D04C-3144
CCATCCCCTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGA
GGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTAAAATTGGCAATCAT
GTCGAAGAGTAACGATCTTTGATTTGGCATAATAATAAGAAGTTGAAGATATGTGTACCT
TCGTTGAGGAGGTAAGCTATCAGAAGCAGATGATCTGCGAGGAGGATTTCTGGACTCAAG
GCTTGATGACGACTCTGTTTCTGAATCTCTCCTTGAACTACTACCCATGCCCTGCGTCAT
GCTCTGAAGAAAAAATTCATTACAAAAAAAAGTTGATCACTACATCTGAGGAGCATACGG
CAAAAACAGATGATAATGGTTTTAACTATGATAGGATGGAAACATGATTCTTAGATGTGA
AGAGAACTTGAGGCTAGGAAATTTGCTAAACGTGTTTTTCAAATCTCTGAAGAACTCTAA
TCTCTACTAAGAGACTTTATTTTTCTTGGAAAACTGAATTTTACTATCTATAACTTCTTC
TCTTGGTTGCGA

Last Updated on 06.12.2018 16:11